School of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi, 530004, China.
Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China.
Food Chem. 2024 Apr 16;438:138044. doi: 10.1016/j.foodchem.2023.138044. Epub 2023 Nov 19.
Nitrite is a carcinogenic substance in food. Excessive consumption of nitrite severely endangers human health. However, rapid and accurate quantification of nitrite by a simple tool is still very challenging. In this work, we designed a practical sensing platform based on 8-(o-phenylenediamine)-boron dipyrromethene (BDP-OPD) to determine nitrite in food. BDP-OPD can take a specific diazotization-cyclization cascade reaction with nitrite to form boron dipyrromethene (BODIPY), giving rise to a remarkable chromogenic reaction along with high contrast fluorescence turn-on response towards nitrite. BDP-OPD has high sensitivity, rapid response, and good selectivity. Furthermore, a portable smartphone-based fluorescence device integrated with a self-programmed Python program was fabricated, which has been successfully used to determine nitrite in food with the advantages of rapid response, low cost, ease of operation, portability, and satisfactory recoveries (92-112%). The good sensing performance rendered BDP-OPD a promising fluorescence platform for on-site visual detection of nitrite.
亚硝酸盐是食品中的致癌物质。过量摄入亚硝酸盐严重危害人类健康。然而，用简单的工具快速准确地定量亚硝酸盐仍然非常具有挑战性。在这项工作中，我们设计了一种基于 8-(邻苯二胺)-硼二吡咯甲川（BDP-OPD）的实用传感平台，用于测定食品中的亚硝酸盐。BDP-OPD 可以与亚硝酸盐发生特定的重氮化-环化级联反应，形成硼二吡咯甲川（BODIPY），从而产生显著的显色反应以及对亚硝酸盐的高对比度荧光开启响应。BDP-OPD 具有高灵敏度、快速响应和良好的选择性。此外，还制备了一种基于智能手机的便携式荧光装置，该装置集成了一个自编的 Python 程序，已成功用于测定食品中的亚硝酸盐，具有快速响应、低成本、易于操作、便携性和令人满意的回收率（92-112%）等优点。BDP-OPD 的良好传感性能使其成为现场可视化检测亚硝酸盐的有前途的荧光平台。
