Poopaibool Nattaporn, Tangprasittipap Amornrat, Chumchuen Sukanya, Satirapod Chonthicha, Singwongsa Artitaya
Reproductive Endocrinology and Infertility Unit, Department of Obstetrics & Gynecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Office of Research, Academic Affairs and Innovations, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Clin Exp Reprod Med. 2023 Dec;50(4):262-269. doi: 10.5653/cerm.2023.06100. Epub 2023 Nov 9.
The aim of this study was to compare semen parameters and sperm DNA fragmentation (SDF) and explore the relationship between semen parameters and SDF between 2 and 7 days of abstinence and a short abstinence period (within 4 hours) in oligozoospermic infertile patients.
Two semen samples were collected from infertile oligozoospermic men (n=34) after an abstinence period of 2 to 7 days and within 4 hours, respectively. Sperm parameters were compared between the two abstinence duration groups, including semen volume, sperm concentration, total sperm count, sperm motility, total motile sperm count (TMSC), morphology, and SDF.
The semen volume, concentration, and total sperm count were significantly decreased after 4 hours of abstinence than after 2 to 7 days of abstinence, with median differences of 1.2 mL (p<0.001), 2×106/mL (p=0.011), and 9.6×106/ejaculation (p<0.001), respectively. TMSC was significantly lower after a short abstinence, with a median difference of 4.24×106/ejaculate (p<0.001). However, there were no significance differences in the percentage of motility, the SDF, and the percentage of sperm with normal morphology. Interestingly, volume, concentration, total sperm count, sperm motility, and SDF, but not TMSC, exhibited significant linear correlations between the two abstinence groups in univariate regression analysis, except for TMSC.
In oligozoospermic men, the volume, concentration, and total sperm count were significantly lower after a short abstinence period, but without adverse effects on sperm motility and SDF.
本研究旨在比较少精子症不育患者禁欲2至7天与短禁欲期（4小时内）的精液参数和精子DNA碎片率（SDF），并探讨精液参数与SDF之间的关系。
分别在禁欲2至7天和4小时内，从34例少精子症不育男性患者中采集两份精液样本。比较两组禁欲时间的精子参数，包括精液量、精子浓度、精子总数、精子活力、总活动精子数（TMSC）、形态和SDF。
禁欲4小时后的精液量、浓度和精子总数显著低于禁欲2至7天后，中位数差异分别为1.2 mL（p<0.001）、2×106/mL（p=0.011）和9.6×106/射精量（p<0.001）。短禁欲后的TMSC显著降低，中位数差异为4.24×106/射精量（p<0.001）。然而，活力百分比、SDF和正常形态精子百分比无显著差异。有趣的是，在单变量回归分析中，除TMSC外，精液量、浓度、精子总数、精子活力和SDF在两组禁欲之间呈现显著的线性相关性。
在少精子症男性中，短禁欲期后的精液量、浓度和精子总数显著降低，但对精子活力和SDF无不良影响。
