The negative effects of the excessive nitrite accumulation raised by anaerobic bioaugmentation on bioremediation of PAH-contaminated soil.

Nitrite accumulation in anaerobic bioaugmentation and its side effects on remediation efficiency of polycyclic aromatic hydrocarbon (PAH)-contaminated soil were investigated in this study. Four gradient doses of PAH-degrading inoculum (10^4, 10^5, 10^6 and 10^7 cells/g soil) were separately supplied to the actual PAH-contaminated soil combining with nitrate as the biostimulant. Although bioaugmented with higher dose of inoculum could effectively improve the biodegradation efficiencies in the initial stage than sole nitrate addition but also accelerated the accumulation of nitrite in soil. The inhibition effects of nitrite swiftly occurred following the rapid accumulation of nitrite in each experiment group, restraining the PAH-degrading functionality by inhibiting the growth of total biomass and denitrifying functions in soil. This study revealed the side effects of nitrite accumulation raised by bioaugmentation on soil microorganisms, contributing to further improving the biodegrading efficiencies in the actual site restoration.