Institute of Agricultural Bio-Environmental Engineering, College of Bio-Systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; The Rural Development Academy, Zhejiang University, Hangzhou 310058, China.
ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 310058, China.
Bioresour Technol. 2024 Feb;393:130049. doi: 10.1016/j.biortech.2023.130049. Epub 2023 Nov 22.
As a novel trend, solid carbon sources are applied to act as electron donors and biofilm carrier in biological denitrification process. In this study, simultaneous nitrate and ammonium removal process in an airlift sequencing batch reactor using 3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) as carbon source and biofilm carrier under intermittent aeration conditions was established to treat effluent of synthetic marine recirculating aquaculture system. The results showed that maximum nitrate and ammonia nitrogen removal rates of 0.45 and 0.09 kg m d were achieved. No significant nitrite accumulation was found during 200-day operation, while effluent dissolved organic carbon accumulation and particle size reduction significantly increased. Microbial community analysis and batch tests illuminate that the generated sludge and attached biofilm played important roles in nitrogen removal. This study demonstrates the potential mechanism for the nitrogen removal process mediated by 3-hydroxybutyrate-co-3-hydroxyvalerate and provide a new idea for the alternative solutions of solid carbon sources.
作为一种新趋势，固体碳源被应用于生物反硝化过程中充当电子供体和生物膜载体。本研究采用 3-羟基丁酸-3-羟基戊酸共聚物（PHBV）作为碳源和生物膜载体，在间歇曝气条件下，在气升式序批式反应器中建立了同时去除硝酸盐和氨氮的工艺，用于处理人工海洋循环水产养殖系统的出水。结果表明，最大硝酸盐和氨氮去除率分别达到 0.45 和 0.09 kg·m d。在 200 天的运行过程中，没有发现明显的亚硝酸盐积累，而出水溶解性有机碳的积累和颗粒尺寸的减小显著增加。微生物群落分析和批量试验表明，生成的污泥和附着的生物膜在脱氮过程中发挥了重要作用。本研究揭示了 3-羟基丁酸-3-羟基戊酸共聚物介导的氮去除过程的潜在机制，为固体碳源的替代解决方案提供了新的思路。
