Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Biological and Environmental Engineering, Cornell University, Riley Robb Hall, Ithaca, NY 14853, USA.
Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Bioresour Technol. 2016 Sep;216:1004-13. doi: 10.1016/j.biortech.2016.06.056. Epub 2016 Jun 17.
In this study, an airlift inner-loop sequencing batch reactor using poly(butylene succinate) as the biofilm carrier and carbon source was operated under an alternant aerobic/anoxic strategy for nitrogen removal in recirculating aquaculture system. The average TAN and nitrate removal rates of 47.35±15.62gNH4-Nm(-3)d(-1) and 0.64±0.14kgNO3-Nm(-3)d(-1) were achieved with no obvious nitrite accumulation (0.70±0.76mg/L) and the dissolved organic carbon in effluents was maintained at 148.38±39.06mg/L. Besides, the activities of dissimilatory nitrate reduction to ammonium and sulfate reduction activities were successfully inhibited. The proteome KEGG analysis illustrated that ammonia might be removed through heterotrophic nitrification, while the activities of nitrate and nitrite reductases were enhanced through aeration treatment. The microbial community analysis revealed that denitrifiers of Azoarcus and Simplicispira occupied the dominate abundance which accounted for the high nitrate removal performance. Overall, this study broadened our understanding of simultaneous nitrification and denitrification using biodegradable material as biofilm carrier.
在这项研究中，使用聚丁二酸丁二醇酯作为生物膜载体和碳源的气升式内环序批式反应器在循环水产养殖系统中采用交替好氧/缺氧策略进行脱氮。在没有明显亚硝酸盐积累（0.70±0.76mg/L）的情况下，实现了平均 47.35±15.62gNH4-Nm(-3)d(-1)的 TAN 和 0.64±0.14kgNO3-Nm(-3)d(-1)的硝酸盐去除率，并且出水的溶解性有机碳保持在 148.38±39.06mg/L。此外，成功抑制了异化硝酸盐还原为铵和硫酸盐还原的活性。KEGG 分析表明，氨可能通过异养硝化去除，而硝酸盐和亚硝酸盐还原酶的活性通过曝气处理得到增强。微生物群落分析表明，脱氮菌属 Azoarcus 和 Simplicispira 占据了优势丰度，这解释了其高硝酸盐去除性能。总的来说，本研究拓宽了我们对使用可生物降解材料作为生物膜载体进行同步硝化反硝化的理解。
