Protein Crystallography Section, Bhabha Atomic Research Centre, Mumbai, 400085, India; Homi Bhabha National Institute, Anushakti Nagar, Mumbai, 400094, India.
Protein Crystallography Section, Bhabha Atomic Research Centre, Mumbai, 400085, India.
Microb Pathog. 2023 Dec;185:106460. doi: 10.1016/j.micpath.2023.106460. Epub 2023 Nov 21.
The COVID-19 pandemic has been driven by the emergence of SARS-CoV-2 variants with mutations across all the viral proteins. Although mutations in the spike protein have received significant attention, understanding the prevalence and potential impact of mutations in other viral proteins is essential for comprehending the evolution of SARS-CoV-2. Here, we conducted a comprehensive analysis of approximately 14 million sequences of SARS-CoV-2 deposited in the GISAID database until December 2022 to identify prevalent mutations in the non-spike proteins at the global and country levels. Additionally, we evaluated the energetics of each mutation to better understand their impact on protein stability. While the consequences of many mutations remain unclear, we discuss potential structural and functional significance of some mutations. Our study highlights the ongoing evolutionary process of SARS-CoV-2 and underscores the importance of understanding changes in non-spike proteins.
COVID-19 大流行是由具有所有病毒蛋白突变的 SARS-CoV-2 变体引起的。尽管刺突蛋白中的突变受到了广泛关注，但了解其他病毒蛋白中的突变的流行程度和潜在影响对于理解 SARS-CoV-2 的进化至关重要。在这里，我们对截至 2022 年 12 月在 GISAID 数据库中存储的约 1400 万条 SARS-CoV-2 序列进行了全面分析，以确定全球和国家层面非刺突蛋白中的常见突变。此外，我们评估了每个突变的能量，以更好地了解它们对蛋白质稳定性的影响。虽然许多突变的后果尚不清楚，但我们讨论了一些突变的潜在结构和功能意义。我们的研究强调了 SARS-CoV-2 的持续进化过程，并强调了了解非刺突蛋白变化的重要性。
