Giannopoulos Athanasios-Stefanos, Giannakou Lydia, Gourgoulianni Natalia, Pitaraki Eleanna, Jagirdar Rajesh, Marnas Periklis, Tzamalas Panagiotis I, Rouka Erasmia, Livanou Eleni, Hatzoglou Chrissi, Gourgoulianis Konstantinos, Lüpold Stefan, Blanckenhorn Wolf U, Zarogiannis Sotirios G
Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Thessaly, BIOPOLIS, 41500 Larissa, Greece.
Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland.
Environ Toxicol Pharmacol. 2023 Nov;104:104325. doi: 10.1016/j.etap.2023.104325. Epub 2023 Nov 22.
Drosophila melanogaster is a widely used animal model in human diseases and to date it has not been applied to the study of the impact of tobacco use on human sexual function. Hence, this report examines the effects of different concentrations of cigarette smoke extract (CSE) exposure on the size and sexual behavior of D. melanogaster. Wild-type flies were held in vials containing CSE-infused culture media at concentrations of 10%, 25%, and 50% for three days, and their offspring were reared under the same conditions before measuring their body size and mating behavior. CSE exposure during development reduced the tibia length and body mass of emerging adult flies and prolonged the time required for successful courtship copulation success, while courtship behaviors (wing extension, tapping, abdomen bending, attempted copulation) remained largely unchanged. Our findings indicate that CSE exposure negatively affects the development of flies and their subsequent reproductive success. Future experiments should investigate the CSE effect on male female fertility.
黑腹果蝇是一种在人类疾病研究中广泛使用的动物模型，迄今为止尚未应用于研究烟草使用对人类性功能的影响。因此，本报告研究了不同浓度香烟烟雾提取物（CSE）暴露对黑腹果蝇体型和性行为的影响。将野生型果蝇置于含有浓度为10%、25%和50%的CSE注入培养基的小瓶中三天，其后代在相同条件下饲养，然后测量其体型和交配行为。发育期间暴露于CSE会降低羽化成年果蝇的胫骨长度和体重，并延长成功求偶交配所需的时间，而求偶行为（翅伸展、轻敲、腹部弯曲、尝试交配）基本保持不变。我们的研究结果表明，暴露于CSE会对果蝇的发育及其随后的繁殖成功率产生负面影响。未来的实验应研究CSE对雄性和雌性生育能力的影响。
