Department of Pharmacy, Children's Hospital of Soochow University, Suzhou, 215000, Jiangsu, China.
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China.
Environ Sci Pollut Res Int. 2023 Dec;30(59):124416-124424. doi: 10.1007/s11356-023-31011-8. Epub 2023 Nov 23.
Chemical composition of the essential oil from Kochia scoparia (L.) Schrad. (syn. Bassia scoparia (L.) A. J. Scott) was analyzed in quality and quantity by GC-MS and GC-FID. Repellent activities of the essential oil from K. scoparia (KSEO) were evaluated against two common species of stored-product insects Tribolium castaneum Herbst and Liposcelis bostrychophila Badonnel. Results indicated that KSEO mainly consisted of eugenol, β-caryophyllene, and α-humulene, accounting for 75.6%, 8.2%, and 1.4% of the total oil, respectively. KSEO and the three major components were repellent to T. castaneum and L. bostrychophila adults. Notably, KSEO exerted significant effects, comparable to the positive control DEET at 2 and 4 h post-exposure. Eugenol at 63.17-2.53 nL/cm exhibited high percentage repellency ranging from 96 to 70% against L. bostrychophila during 4-h exposure. To gain further insights into the repellent activity, molecular docking simulation was performed with eugenol as the ligand and an odorant binding protein TcOBPC12 (gene: TcOBP10B) from the model insect T. castaneum as the receptor. Docking calculation results revealed that TcOBPC12 had binding affinity to eugenol (△G = - 4.52 kcal/mol) along with a hydrogen bond of 0.18 nm (1.8 Å) long forming between them, which could be an important target protein associated with identifying volatile repellent molecules. This work highlights the promising potential of KSEO as a botanical repellent for controlling stored-product insects.
苦参（Kochia scoparia（L.）Schrad.）（同义词：Bassia scoparia（L.）A. J. Scott）精油的化学成分通过 GC-MS 和 GC-FID 进行了定性和定量分析。评价了苦参精油（KSEO）对两种常见的仓储害虫赤拟谷盗（Tribolium castaneum Herbst）和嗜卷书虱（Liposcelis bostrychophila Badonnel）的驱避活性。结果表明，KSEO 主要由丁香酚、β-石竹烯和α-葎草烯组成，分别占总油量的 75.6%、8.2%和 1.4%。KSEO 和三种主要成分对赤拟谷盗和嗜卷书虱成虫均具有驱避作用。值得注意的是，KSEO 在暴露后 2 和 4 小时的效果与阳性对照 DEET 相当。丁香酚在 63.17-2.53 nL/cm 时对嗜卷书虱在 4 小时暴露期间表现出 96%至 70%的高百分比驱避率。为了进一步了解驱避活性，以丁香酚为配体，以模式昆虫赤拟谷盗的气味结合蛋白 TcOBPC12（基因：TcOBP10B）为受体，进行了分子对接模拟。对接计算结果表明，TcOBPC12 与丁香酚（△G = -4.52 kcal/mol）具有结合亲和力，它们之间形成 0.18nm（1.8Å）长的氢键，这可能是与识别挥发性驱避分子相关的重要靶蛋白。这项工作突出了 KSEO 作为一种植物性驱避剂控制仓储害虫的巨大潜力。
