Guo Shanshan, Zhang Wenjuan, Liang Junyu, You Chunxue, Geng Zhufeng, Wang Chengfang, Du Shushan
Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China.
Analytical and Testing Center, Beijing Normal University, Beijing 100875, China.
Molecules. 2016 Apr 16;21(4):504. doi: 10.3390/molecules21040504.
The chemical composition of the essential oil from Juniperus formosana leaves and its contact and repellent activities against Tribolium castaneum and Liposcelis bostrychophila adults were investigated. The essential oil of J. formosana leaves was obtained by hydrodistillation and analyzed by GC-MS. A total of 28 components were identified and the main compounds in the essential oil were α-pinene (21.66%), 4-terpineol (11.25%), limonene (11.00%) and β-phellandrene (6.63%). The constituents α-pinene, 4-terpineol and d-limonene were isolated from the essential oil. It was found that the essential oil exhibited contact activity against T. castaneum and L. bostrychophila adults (LD50 = 29.14 μg/adult and 81.50 µg/cm², respectively). The compound 4-terpineol exhibited the strongest contact activity (LD50 = 7.65 μg/adult). In addition, data showed that at 78.63 nL/cm², the essential oil and the three isolated compounds strongly repelled T. castaneum adults. The compounds α-pinene and d-limonene reached the same level (Class V) of repellency as DEET (p = 0.396 and 0.664) against L. bostrychophila at 63.17 nL/cm² after 2 h treatment. The results indicate that the essential oil and the isolated compounds have potential to be developed into natural insecticides and repellents to control insects in stored products.
对台湾桧叶精油的化学成分及其对赤拟谷盗和嗜卷书虱成虫的触杀和驱避活性进行了研究。采用水蒸馏法提取台湾桧叶精油，并通过气相色谱-质谱联用仪进行分析。共鉴定出28种成分，精油中的主要化合物为α-蒎烯（21.66%）、4-松油醇（11.25%）、柠檬烯（11.00%）和β-水芹烯（6.63%）。从精油中分离出了α-蒎烯、4-松油醇和d-柠檬烯成分。结果发现，该精油对赤拟谷盗和嗜卷书虱成虫具有触杀活性（LD50分别为29.14 μg/成虫和81.50 µg/cm²）。化合物4-松油醇表现出最强的触杀活性（LD50 = 7.65 μg/成虫）。此外，数据表明，在78.63 nL/cm²时，该精油及其三种分离出的化合物对赤拟谷盗成虫具有强烈的驱避作用。在处理2小时后，化合物α-蒎烯和d-柠檬烯在63.17 nL/cm²时对嗜卷书虱的驱避效果达到了与避蚊胺相同的水平（V级）（p = 0.396和0.664）。结果表明，该精油及其分离出的化合物有潜力被开发成天然杀虫剂和驱避剂，用于防治储藏物中的害虫。
