Laboratoire de Microbiologie et Biologie Moléculaire, Centre de Biotechnologie Végétale et Microbienne Biodiversité et Environnement, Faculté des Sciences, Université Mohammed V, 10000, Rabat, Morocco.
Centre d'Agrobiotechnologie et Bioingénierie, Unité de Recherche Labellisée CNRST (AgroBiotech-URL-CNRST 05), Faculté des Sciences et Techniques, Université Cadi Ayyad, 40000, Marrakech, Morocco.
Environ Sci Pollut Res Int. 2023 Dec;30(59):124263-124273. doi: 10.1007/s11356-023-31103-5. Epub 2023 Nov 24.
Phosphorus (P) is a vital limiting nutrient element for plant growth and yield. In Morocco, the natural phosphate rock extractions generate significant amounts of phosphate wash sludge (PS), which could be reused productively, thus creating another added value for farmers. The present study aimed to demonstrate the combination effect of soil amendment by two different PS concentrations (1% and 5%) associated with three phosphate-solubilizing bacteria (PSB) consortia (C1, C2, and C3), isolated from phosphate mining sludge, on plant growth and nutrient uptake in tomato seedlings (Solanum lycopersicum). The results obtained showed that this bioformulation significantly improved P solubilization and plant growth compared to control conditions. Of all the combinations, C3-inoculated soil amended with 5% PS was the most effective in significantly improving plant height and dry and fresh biomass of shoots and roots. P solubilization and its availability for tomato seedlings uptake were maximal with the bioformulation (C3 + 5% PS). This latter enhanced P and potassium (K) uptake by 27.89 and 38.81% in shoots and 38.57% and 74.67% in roots, respectively, compared to non-inoculated soil amended with 5% PS. The highest flowering rate (200 %) was recorded in C3-inoculated soil amended with 5% PS. Supporting these results, the principal component analysis discriminated this bioformulation (C3 + 5% PS) from the other combinations. Our results open up prospects for upgrading phosphate sludge enriched with PSB consortia as a biofertilizer that can be used in ecofriendly agriculture integrated into the circular economy.
磷（P）是植物生长和产量的重要限制养分元素。在摩洛哥，天然磷矿开采产生了大量的磷洗泥（PS），这些磷洗泥可以被重新利用，从而为农民创造另一个附加值。本研究旨在展示两种不同 PS 浓度（1%和 5%）与三种从磷矿泥中分离的解磷菌（PSB）菌群（C1、C2 和 C3）的土壤改良相结合对番茄幼苗（Solanum lycopersicum）生长和养分吸收的组合效应。结果表明，与对照条件相比，这种生物制剂显著提高了 P 的溶解和植物生长。在所有组合中，用 5%PS 改良的接种 C3 的土壤在显著提高株高和茎、根干鲜生物量方面最为有效。生物制剂（C3+5%PS）使 P 溶解及其对番茄幼苗吸收的有效性达到最大值。与未接种土壤相比，该生物制剂分别提高了 27.89%和 38.81%的地上部和 38.57%和 74.67%的根部 P 和钾（K）的吸收。在接种 C3 的土壤中，用 5%PS 改良的土壤的开花率最高（200%）。主成分分析将该生物制剂（C3+5%PS）与其他组合区分开来，支持了这些结果。我们的研究结果为升级富含 PSB 菌群的磷矿泥作为生物肥料开辟了前景，这种生物肥料可用于环保农业，融入循环经济。
