Bakki Mohamed, Banane Badra, Marhane Omaima, Esmaeel Qassim, Hatimi Abdelhakim, Barka Essaid Ait, Azim Khalid, Bouizgarne Brahim
Laboratory of Plant Biotechnology "Biotechnologies Végétales", Faculty of Sciences, University Ibn Zohr (UIZ), Agadir, Morocco.
Unité de Recherche Résistance Induite et Bio Protection des Plantes, EA 4707 - USC INRAe1488, UFR Sciences Exactes et Naturelles, Moulin de la Housse, University of Reims Champagne-Ardenne, Reims, France.
Front Microbiol. 2024 May 3;15:1289466. doi: 10.3389/fmicb.2024.1289466. eCollection 2024.
Nowadays, sustainable agriculture approaches are based on the use of biofertilizers and biopesticides. Tomato ( L.) rhizosphere could provide rhizobacteria with biofertilizing and biopesticide properties. In this study, bacteria from the rhizosphere of tomato were evaluated for plant growth promotion (PGP) properties. Five isolates (PsT-04c, PsT-94s, PsT-116, PsT-124, and PsT-130) and one isolate (BaT-68s), with the highest ability to solubilize tricalcium phosphate (TCP) were selected for further molecular identification and characterization. Isolates showed phosphate solubilization up to 195.42 μg mL. All isolates showed phosphate solubilization by organic acid production. The six isolates improved seed germination and showed effective root colonization when tomato seeds were coated with isolates at 10 cfu g in axenic soil conditions. Furthermore, the selected isolates were tested for beneficial effects on tomato growth and nutrient status in greenhouse experiments with natural rock phosphate (RP). The results showed that inoculated tomato plants in the presence of RP have a higher shoot and root lengths and weights compared with the control. After 60 days, significant increases in plant Ca, Na, P, protein, and sugar contents were also observed in inoculated seedlings. In addition, inoculated tomato seedlings showed an increase in foliar chlorophyll a and b and total chlorophyll, while no significant changes were observed in chlorophyll fluorescence. In greenhouse, two isolates, PsT-04c and PsT-130, showed ability to trigger induced systemic resistance in inoculated tomato seedlings when subsequently challenged by subsp. , the causal agent of tomato bacterial canker. High protection rate (75%) was concomitant to an increase in the resistance indicators: total soluble phenolic compounds, phenylalanine-ammonia lyase, and HO. The results strongly demonstrated the effectiveness of phosphate-solubilizing bacteria adapted to rhizosphere as biofertilizers for tomato crops and biopesticides by inducing systemic resistance to the causal agent of tomato bacterial canker disease.
如今，可持续农业方法基于生物肥料和生物农药的使用。番茄（L.）根际可为具有生物肥料和生物农药特性的根际细菌提供生存环境。在本研究中，对番茄根际细菌的植物生长促进（PGP）特性进行了评估。选择了五株溶磷能力最强的菌株（PsT - 04c、PsT - 94s、PsT - 116、PsT - 124和PsT - 130）和一株菌株（BaT - 68s）进行进一步的分子鉴定和表征。这些菌株的溶磷量高达195.42μg/mL。所有菌株均通过产生有机酸来溶解磷酸盐。当在无菌土壤条件下用10 cfu/g的菌株包衣番茄种子时，这六株菌株均能提高种子发芽率并有效定殖于根部。此外，在添加天然磷矿粉（RP）的温室试验中，对所选菌株对番茄生长和养分状况的有益作用进行了测试。结果表明，与对照相比，在添加RP的情况下接种番茄植株的地上部和根部长度及重量更高。60天后，接种幼苗的植株钙、钠、磷、蛋白质和糖含量也显著增加。此外，接种的番茄幼苗叶片叶绿素a和b以及总叶绿素含量增加，而叶绿素荧光未观察到显著变化。在温室中，当随后受到番茄溃疡病菌亚种挑战时，两株菌株PsT - 04c和PsT - 130显示出能够在接种的番茄幼苗中引发诱导系统抗性。高保护率（75%）伴随着抗性指标的增加：总可溶性酚类化合物、苯丙氨酸解氨酶和HO。结果有力地证明了适应根际环境的溶磷细菌作为番茄作物生物肥料和通过诱导对番茄溃疡病病原菌的系统抗性作为生物农药的有效性。
