State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China.
College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China.
J Agric Food Chem. 2023 Dec 6;71(48):19033-19044. doi: 10.1021/acs.jafc.3c07269. Epub 2023 Nov 23.
Starch-lipid-protein complexes are attracting increasing attention due to their unique structure and low enzymatic digestibility. However, the mechanisms underlying the formation of these ternary complexes, especially those with monoglycerides as the lipid component, remain unclear. In the present study, potato starch or octenyl succinic anhydride (OSA)-modified potato starch (OSAPS), various monoglycerides (MGs), and beta-lactoglobulin (βLG) were used in model systems to characterize the formation, structure, and in vitro digestibility of the respective ternary complexes. Colorimetry and live/dead staining assays demonstrated that the OSAPS had good biocompatibility. Experimental data and molecular dynamics simulations showed that both unmodified potato starch and OSAPS formed starch-lipid-protein complexes with MGs and βLG. Of the two types of starch, OSA formed a greater amount of the more stable type II V-crystallites in complexes, which had greater resistance to in vitro enzymic digestion. This study demonstrated for the first time that starch can interact with MGs and βLG to form ternary complexes and that OSA esterification of starch promoted the formation of more complexes than unmodified starch.
淀粉-脂质-蛋白质复合物由于其独特的结构和低酶消化性而受到越来越多的关注。然而，这些三元复合物形成的机制，特别是那些以单甘油脂作为脂质成分的三元复合物形成的机制仍不清楚。在本研究中，使用马铃薯淀粉或辛烯基琥珀酸酐（OSA）修饰的马铃薯淀粉（OSAPS）、各种单甘油脂（MGs）和β-乳球蛋白（βLG）在模型体系中，对各自的三元复合物的形成、结构和体外消化性进行了表征。比色法和死活染色试验表明 OSAPS 具有良好的生物相容性。实验数据和分子动力学模拟表明，未修饰的马铃薯淀粉和 OSAPS 均与 MGs 和βLG 形成淀粉-脂质-蛋白质复合物。在这两种淀粉中，OSA 在复合物中形成了更多的更稳定的 II 型 V 型微晶，其对体外酶消化的抵抗力更强。本研究首次证明淀粉可以与 MGs 和βLG 相互作用形成三元复合物，并且淀粉的 OSA 酯化作用比未修饰的淀粉促进了更多复合物的形成。
