Jiang Weijia, He Ran, Lv Han, He Xinheng, Wang Li, Wei Yanli
Institute of Environmental Science, Shanxi University, Taiyuan 030031, China.
The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Research Center of Neurobiology, Shanxi University of Chinese Medicine, Jinzhong 030619, China.
ACS Sens. 2023 Nov 24;8(11):4264-4271. doi: 10.1021/acssensors.3c01616. Epub 2023 Nov 2.
Chiral recognition of amino acid plays a significant role in pharmaceutical, medical, and food science. This study describes a chiral sensing system of β-cyclodextrin (β-CD)-coated sulfur quantum dots (CD-SQDs) for the selective fluorescence recognition of tryptophan (Trp) enantiomers. CD-SQDs were prepared by a facile assembly fission method and could selectively recognize -Trp by the different binding ability between /-Trp and β-CD. The inclusion of -Trp and the stereoselective catalysis of CD-SQDs enzyme mimics cause the increased fluorescence intensity of CD-SQDs, which has a linear response ranging from 10 to 500 nM and the detection limit as 2.3 nM. CD-SQDs also show great selectivity for -Trp from the commercial compound amino acid injection. The study could provide an effective method for the chiral recognition of amino acid enantiomers based on the catalytic activity of nanoenzymes.
氨基酸的手性识别在制药、医学和食品科学中发挥着重要作用。本研究描述了一种用于色氨酸（Trp）对映体选择性荧光识别的β-环糊精（β-CD）包覆硫量子点（CD-SQDs）手性传感系统。CD-SQDs通过简便的组装裂变方法制备，可通过/-Trp与β-CD之间不同的结合能力选择性识别-Trp。-Trp的包合以及CD-SQDs模拟酶的立体选择性催化导致CD-SQDs的荧光强度增加，其线性响应范围为10至500 nM，检测限为2.3 nM。CD-SQDs对市售复方氨基酸注射液中的-Trp也表现出良好的选择性。该研究可为基于纳米酶催化活性的氨基酸对映体手性识别提供一种有效方法。
