School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 15;323:124871. doi: 10.1016/j.saa.2024.124871. Epub 2024 Jul 29.
Different enantiomer forms of amino acids play different roles in multifarious fields, and improper use will cause irreversible effects. Therefore, the identification of chiral amino acids is a vital issue in the field of pharmaceutical analysis. Herein, a chiral sensing system of β-cyclodextrin coated silver nanoparticle (β-CD@AgNPs) with peroxidase-like activity was designed for the fast and efficient colorimetric identification of tryptophan (Trp) enantiomers based on the difference in binding capacity between D/L-Trp and β-CD. The results showed the satisfactory linearity for detecting D/L-Trp over the concentration range from 0.2 to 4 mM with a LOD of 0.16 and 0.18 mM, respectively. Moreover, the absorbance increased linearly with the rise of D-Trp concentration percentage in the Trp enantiomer mixture. The proposed method avoided the use of natural enzymes and improved the stability due to the protective effect of cyclodextrin, which provided a new idea for selective colorimetric recognition and detection of D/L-Trp based on cyclodextrin.
不同的氨基酸对映体形式在众多领域中发挥着不同的作用，使用不当会造成不可逆转的影响。因此，手性氨基酸的鉴定是药物分析领域的一个重要问题。本文设计了一种具有过氧化物酶样活性的β-环糊精包覆的银纳米粒子（β-CD@AgNPs）手性传感体系，基于 D/L-色氨酸与β-CD 之间结合能力的差异，实现了对色氨酸对映异构体的快速、高效比色识别。结果表明，该方法对 D/L-色氨酸的检测具有令人满意的线性范围，在 0.2 至 4 mM 浓度范围内，检测限分别为 0.16 和 0.18 mM。此外，随着色氨酸对映异构体混合物中 D-色氨酸浓度百分比的升高，吸光度呈线性增加。该方法避免了使用天然酶，并由于环糊精的保护作用提高了稳定性，为基于环糊精的 D/L-色氨酸的选择性比色识别和检测提供了新的思路。
