Alkudmani Zeina S, Alzailai Aminah Ahmad, Aburisheh Khaled H, Alshammary Amal F, Ali Khan Imran
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia.
University Diabetes Center, King Saud University Medical City, King Saud University, Riyadh 11472, Saudi Arabia.
Biology (Basel). 2023 Nov 16;12(11):1439. doi: 10.3390/biology12111439.
Diabetes mellitus is a complex disease with a wide range of manifestations. Diabetes, notably type 2 diabetes mellitus (T2DM), is becoming more common in Saudi Arabia as a result of obesity and an aging population. T2DM is classified as a noncommunicable disease, and its incidence in the Saudi population continues to grow as a consequence of socioeconomic changes. Toll-like receptors (TLRs) are innate immune receptors that mediate the inflammatory response in diabetes mellitus. Previous studies have documented the relationship between different SNPs in the gene in different forms of diabetes. As a result, the purpose of this study was to investigate the relationship between rs187084, rs352140, and rs5743836 SNPs in the gene among T2DM patients in the Saudi population. This was a case-control study that included 100 T2DM cases and 100 control subjects. The three SNPs were identified in the study population (n = 200) using polymerase chain reaction (PCR), restriction enzymes for rs352140, and Sanger sequencing for rs187084 and rs5783836. Next, statistical analyses were performed using various software to determine the association between the SNPs and T2DM. rs187084 and rs5743836 were associated with an increased risk of T2DM development. rs187084 and rs5743836 allelic frequencies were associated with a 3.2 times increased risk of T2DM development ( < 0.05). DBP was associated with T2DM ( = 0.02). rs187084 was associated with TC and HDLc; rs352140 was associated with DBP, HbA1c, and HDLc; rs5743836 was associated with waist ( < 0.05). The CGT haplotype was strongly associated with T2DM ( < 0.003). Gene-gene interaction, graphical presentation, and dendrogram showed the strong association with T2DM patients ( < 0.05). This study concluded that rs187084 and rs5743836 were strongly associated with T2DM in Saudi Arabian patients. This study provides further evidence that SNPs in the gene play a significant role in T2DM development in a Saudi community.
糖尿病是一种具有广泛临床表现的复杂疾病。糖尿病，尤其是2型糖尿病（T2DM），在沙特阿拉伯因肥胖和人口老龄化而变得越来越普遍。T2DM被归类为非传染性疾病，由于社会经济变化，其在沙特人群中的发病率持续上升。Toll样受体（TLRs）是介导糖尿病炎症反应的固有免疫受体。先前的研究记录了该基因中不同单核苷酸多态性（SNPs）与不同类型糖尿病之间的关系。因此，本研究的目的是调查沙特人群中T2DM患者该基因中rs187084、rs352140和rs5743836 SNPs之间的关系。这是一项病例对照研究，包括100例T2DM病例和100名对照受试者。使用聚合酶链反应（PCR）、rs352140的限制性内切酶以及rs187084和rs5783836的桑格测序法在研究人群（n = 200）中鉴定了这三个SNPs。接下来，使用各种软件进行统计分析，以确定SNPs与T2DM之间的关联。rs187084和rs5743836与T2DM发生风险增加相关。rs187084和rs5743836的等位基因频率与T2DM发生风险增加3.2倍相关（P < 0.05）。舒张压（DBP）与T2DM相关（P = 0.02）。rs187084与总胆固醇（TC）和高密度脂蛋白胆固醇（HDLc）相关；rs352140与DBP、糖化血红蛋白（HbA1c）和HDLc相关；rs5743836与腰围相关（P < 0.05）。CGT单倍型与T2DM密切相关（P < 0.003）。基因-基因相互作用、图形展示和树状图显示与T2DM患者密切相关（P < 0.05）。本研究得出结论，rs187084和rs5743836与沙特阿拉伯患者的T2DM密切相关。本研究提供了进一步的证据，表明该基因中的SNPs在沙特社区T2DM的发生中起重要作用。
