Ghosheh Nour, Naffa Randa G, Mashal Safaa, Al-Khalayfa Sawsan, Battah AbdelKader Hamdi, Azab Bilal
Department of Pathology, Microbiology and Forensic Medicine, School of Medicine, University of Jordan, Queen Rania St, PO Box: 13617, Amman, 11942, Jordan.
Department of Basic Dental Sciences, Faculty of Dentistry, Al-Ahliyya Amman University, Amman, Jordan.
Mol Biol Rep. 2025 May 16;52(1):461. doi: 10.1007/s11033-025-10544-w.
Type 2 diabetes mellitus (T2D) is one of the biggest health concerns of our time. T2D prevalence is expected to reach 1.9 million Jordanians by 2050. Owing to this sharp increase, Jordanians should understand genetic risk factors for this disease. One of the strongest reported single nucleotide polymorphisms (SNPs) associated with T2D is located in the transcription factor 7-like 2 (TCF7L2) gene, particularly rs12255372 and rs7903146. Despite this, contradictory results suggesting no association with T2D were found across ethnicities. Therefore, this study investigated the association of rs12255372 and rs7903146 with T2D in Jordanians.
For this case-control study, 301 non-diabetic healthy controls and 301 patients with T2D were genotyped for rs12255372 and rs7903146 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A significant development in T2D was demonstrated in rs12255372 and rs7903146. The risk allele for both single nucleotide polymorphisms is the T allele. The p-value ≤ 0.001 reflects a significant difference between the control group and the T2D group. Moreover, the combined effect of rs12255372 and rs7903146 showed statistical significance with a p-value ≤ 0.001.
Our study suggests that TCF7L2 gene polymorphisms (rs12255372 and rs7903146) are predisposing risk factors for T2D in the Jordanian population.
2型糖尿病（T2D）是我们这个时代最大的健康问题之一。预计到2050年，约旦患2型糖尿病的人数将达到190万。由于这一急剧增长，约旦人应该了解这种疾病的遗传风险因素。与2型糖尿病相关的最强报道的单核苷酸多态性（SNP）之一位于转录因子7样2（TCF7L2）基因中，特别是rs12255372和rs7903146。尽管如此，在不同种族中发现了与之矛盾的结果，表明与2型糖尿病无关。因此，本研究调查了rs12255372和rs7903146与约旦人2型糖尿病的关联。
在这项病例对照研究中，使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对301名非糖尿病健康对照者和301名2型糖尿病患者进行了rs12255372和rs7903146基因分型。在rs12255372和rs7903146中显示出2型糖尿病的显著发展。这两个单核苷酸多态性的风险等位基因都是T等位基因。p值≤0.001反映了对照组和2型糖尿病组之间的显著差异。此外，rs12255372和rs7903146的联合效应显示出统计学意义，p值≤0.001。
我们的研究表明，TCF7L2基因多态性（rs12255372和rs7903146）是约旦人群中2型糖尿病的易感风险因素。
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