Zhong Yuying, Zheng Wenhuang, Shi Xinguo, Guo Yisong, Wang Qianqian, Lv Pin, Chen Jianfeng
Technical Innovation Service Platform for High Value and High Quality Utilization of Marine Organism, Fuzhou University, Fuzhou 350108, China.
Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste, Fuzhou University, Fuzhou 350108, China.
Biology (Basel). 2023 Nov 17;12(11):1447. doi: 10.3390/biology12111447.
The role of microorganisms in effectively terminating harmful algal blooms (HABs) is crucial for maintaining environmental stability. Recent studies have placed increased emphasis on bio-agents capable of inhibiting HABs. The bacterium sp. strain FDHY-MZ2 has exhibited impressive algicidal abilities against , a notorious global HAB-forming species. To augment this capability, cultures were progressively scaled from shake flask conditions to small-scale (5 L) and pilot-scale (50 L) fermentation. By employing a specifically tailored culture medium (2216E basal medium with 1.5% soluble starch and 0.5% peptone), under precise conditions (66 h, 20 °C, 450 rpm, 30 L/min ventilation, 3% seeding, and constant starch flow), a notable increase in algicidal bacterial biomass was observed; the bacterial dosage required to entirely wipe out within a day decreased from 1% to 0.025%. Compared to an unoptimized shake flask group, the optimized fermentation culture caused significant reductions in algal chlorophyll and protein levels (21.85% and 78.3%, respectively). Co-culturing induced increases in algal malondialdehyde and HO by 5.98 and 5.38 times, respectively, leading to further disruption of algal photosynthesis. This study underscores the unexplored potential of systematically utilized microbial agents in mitigating HABs, providing a pathway for their wider application.
微生物在有效终止有害藻华（HABs）方面的作用对于维持环境稳定性至关重要。最近的研究越来越重视能够抑制有害藻华的生物制剂。细菌sp.菌株FDHY-MZ2已表现出对一种全球臭名昭著的形成有害藻华的物种的显著杀藻能力。为了增强这种能力，培养物逐步从摇瓶条件扩大到小规模（5升）和中试规模（50升）发酵。通过使用专门定制的培养基（含有1.5%可溶性淀粉和0.5%蛋白胨的2216E基础培养基），在精确条件下（66小时、20°C、450转/分钟、30升/分钟通气、3%接种量和恒定淀粉流），观察到杀藻细菌生物量显著增加；一天内完全消灭所需的细菌剂量从1%降至0.025%。与未优化的摇瓶组相比，优化后的发酵培养导致藻类叶绿素和蛋白质水平显著降低（分别降低21.85%和78.3%）。共培养分别使藻类丙二醛和HO增加5.98倍和5.38倍，导致藻类光合作用进一步受到破坏。这项研究强调了系统利用微生物制剂在减轻有害藻华方面尚未被探索的潜力，为其更广泛的应用提供了一条途径。
