Buschmann Christina, Unverdorben Laura, Knabl Julia, Hutter Stefan, Meister Sarah, Beyer Susanne, Burgmann Maximiliane, Keilmann Lucia, Zati Zehni Alaleh, Schmoeckel Elisa, Kessler Mirjana, Jeschke Udo, Mahner Sven, Kolben Thomas, Ganster Franziska, Burges Alexander
Department of Obstetrics and Gynecology, University Hospital, LMU Munich, 81377 Munich, Germany.
Department of Pathology, University Hospital, LMU Munich, 81377 Munich, Germany.
Curr Issues Mol Biol. 2023 Nov 2;45(11):8840-8851. doi: 10.3390/cimb45110554.
Galectins are known to play an important role in immunoregulatory processes and autoimmune diseases. Galectin-10 is a cytoplasmic protein of human eosinophils and is involved in various eosinophilic diseases. Since increased galectin expression is already detected in the placentas of mothers with gestational diabetes mellitus (GDM), this study focuses on the specific role of galectin-10 and hints at consequences for the diagnosis and therapeutic options of GDM. It is hypothesized that the difference in galectin-10 expression will raise the pathophysiological understanding of gestational diabetes. The study population consists of 80 women: 40 healthy mothers and 40 women suffering from gestational diabetes mellitus. The expression of galectin-10 was analyzed in the syncytiotrophoblast (SCT) and the decidua of the placenta via immunohistochemistry and immunofluorescence double staining. The immunoreactivity score (IRS) was used for evaluation. The results in this study were significant for an overexpression of galectin-10 in GDM placentas compared with the control group. The syncytiotrophoblast showed overexpression in the nucleus and the cytoplasm, whereas expression of galectin-10 in the decidua was significant in the cytoplasm only. This study identified the expression changes in galectin-10 in placental tissue between healthy and GDM mothers and intensified the understanding of gestational diabetes. Assuming that gestational diabetes mellitus is involved in inflammatory processes, galectin-10 might play a role in the development and maintenance of GDM. Further investigation is required to strengthen these findings.
已知半乳糖凝集素在免疫调节过程和自身免疫性疾病中发挥重要作用。半乳糖凝集素-10是人类嗜酸性粒细胞的一种细胞质蛋白，参与多种嗜酸性粒细胞疾病。由于在患有妊娠期糖尿病（GDM）的母亲的胎盘中已检测到半乳糖凝集素表达增加，本研究聚焦于半乳糖凝集素-10的具体作用，并暗示其对GDM诊断和治疗选择的影响。据推测，半乳糖凝集素-10表达的差异将提高对妊娠期糖尿病的病理生理学认识。研究人群包括80名女性：40名健康母亲和40名患有妊娠期糖尿病的女性。通过免疫组织化学和免疫荧光双重染色分析胎盘合体滋养层（SCT）和蜕膜中半乳糖凝集素-10的表达。免疫反应性评分（IRS）用于评估。本研究结果显示，与对照组相比，GDM胎盘组织中半乳糖凝集素-10过表达具有统计学意义。合体滋养层在细胞核和细胞质中均有过表达，而半乳糖凝集素-10在蜕膜中的表达仅在细胞质中显著。本研究确定了健康母亲和GDM母亲胎盘组织中半乳糖凝集素-10的表达变化，加深了对妊娠期糖尿病的理解。假设妊娠期糖尿病参与炎症过程，半乳糖凝集素-10可能在GDM的发生和维持中发挥作用。需要进一步研究以强化这些发现。
