Machado Daniela, Fonseca Mariana, Vedor Rita, Sousa Sérgio, Barbosa Joana Cristina, Gomes Ana Maria
Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Gels. 2023 Nov 1;9(11):869. doi: 10.3390/gels9110869.
is considered a next-generation probiotic to be incorporated in new food and pharmaceutical formulations. Effective delivery systems are required to ensure high probiotic viability and stability during product manufacture, shelf-life, and post-consumption, namely, throughout digestion. Hydrogelated matrices have demonstrated promising potential in this dominion. Hence, this work aimed to evaluate the effect of a calcium-alginate hydrogelated matrix on viability during 28-days refrigerated aerobic storage and when exposed to simulated gastrointestinal conditions, in comparison with that of free cells. was successfully encapsulated in the calcium-alginate matrix via extrusion (60% encapsulation yield). Furthermore, encapsulated exhibited high stability (a loss in viability lower than 0.2 log-cycle) after 28-days of refrigerated aerobic storage, maintaining its viability around 10 CFU/g. Prominently, as the storage time increased, encapsulated revealed higher viability and stability regarding in vitro gastrointestinal conditions than free cells. This suggests that this encapsulation method may attenuate the detrimental effects of prolonged aerobic storage with a subsequent gastrointestinal passage. In conclusion, encapsulation via extrusion using a calcium-alginate hydrogelated matrix seems to be a promising and adequate strategy for safeguarding from adverse conditions encountered during refrigerated aerobic storage and when exposed to the gastrointestinal passage.
被认为是一种可纳入新食品和药物制剂的下一代益生菌。需要有效的递送系统来确保益生菌在产品制造、保质期和消费后（即整个消化过程）中的高活力和稳定性。水凝胶化基质在这一领域已显示出有前景的潜力。因此，本研究旨在评估与游离细胞相比，海藻酸钙水凝胶化基质对其在28天冷藏需氧储存期间以及暴露于模拟胃肠道条件下的活力的影响。通过挤压成功地将其包封在海藻酸钙基质中（包封率为60%）。此外，包封后的在28天冷藏需氧储存后表现出高稳定性（活力损失低于0.2个对数循环），其活力维持在10 CFU/g左右。显著的是，随着储存时间的增加，包封后的在体外胃肠道条件下比游离细胞表现出更高的活力和稳定性。这表明这种包封方法可能减轻延长的需氧储存以及随后胃肠道通过所带来的有害影响。总之，使用海藻酸钙水凝胶化基质通过挤压进行包封似乎是一种有前景且合适的策略，可保护免受冷藏需氧储存期间以及暴露于胃肠道通过时所遇到的不利条件的影响。
