Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, Shaanxi, China.
State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Xianyang, Shaanxi, China..
Int J Biol Macromol. 2020 Sep 15;159:373-382. doi: 10.1016/j.ijbiomac.2020.05.055. Epub 2020 May 15.
We explored the possibility of improving the viability of Akkermansia muciniphila by encapsulating it in succinate-grafted alginate doped with epigallocatechin-3-gallate (EGCG). In this study, the determined surface properties of microcapsules and modified materials and the measured viability of probiotics after spray drying showed that the modified sodium alginate made the surfaces of microcapsules smoother and denser during the spray drying, thus preventing damages. EGCG enhanced the antioxidant capacity of probiotics by filling the pores inside microgels. Moreover, we analyzed the long-term storage vitality changes, oxidation resistance, uniformity, particle size and Zeta potential of microcapsules and found that spray-dried modified sodium alginate microcapsules with EGCG showed the better storability and stability. In addition, we experimentally analyzed the resistances of different microcapsules to the gastrointestinal fluid and found that EGCG-modified sodium alginate microcapsules better protected the probiotic activity from gastrointestinal fluid. This study provides a slimming product with industrial application potential.
我们探索了通过将 Akkermansia muciniphila 包封在琥珀酸接枝的海藻酸钠中并掺杂表没食子儿茶素没食子酸酯 (EGCG) 来提高其存活率的可能性。在这项研究中，微胶囊和改性材料的确定表面特性以及喷雾干燥后益生菌的测定存活率表明，改性海藻酸钠在喷雾干燥过程中使微胶囊的表面更加光滑和致密，从而防止了损伤。EGCG 通过填充微凝胶内部的孔隙增强了益生菌的抗氧化能力。此外，我们分析了微胶囊的长期储存活力变化、抗氧化性、均匀性、粒径和 Zeta 电位，发现含有 EGCG 的喷雾干燥改性海藻酸钠微胶囊具有更好的储存稳定性和稳定性。此外，我们通过实验分析了不同微胶囊对胃肠道液的抵抗力，发现 EGCG 改性海藻酸钠微胶囊能更好地保护益生菌活性免受胃肠道液的影响。这项研究提供了一种具有工业应用潜力的减肥产品。
