Wilhelm J, Vilím V
Anal Biochem. 1986 Oct;158(1):201-10. doi: 10.1016/0003-2697(86)90610-x.
We tested the effects of generally used chemiluminescence inhibitors on an example of luminol chemiluminescence elicited by xanthine oxidase/hypoxanthine system, and attempted to assess their capabilities in discovering the reaction pathways leading to chemiluminescence. Luminol itself is a xanthine oxidase inhibitor and its concentration affects the reaction mechanism. Maximal chemiluminescence response was observed at luminol concentration inhibiting urate production. Chemiluminescence was totally inhibited by superoxide dismutase, the inhibition by catalase depended on luminol concentration. Ferricytochrome c, a detector of superoxide, either stimulated or inhibited chemiluminescence in a concentration-dependent manner. Chemiluminescence was highly stimulated by peroxidases. A pronounced inhibition of chemiluminescence was caused by chelators; 1 mM desferal and 0.01 mM diethyldithiocarbamate. It is suggested that measurement of luminol chemiluminescence is not a suitable method for discrimination among individual reactive oxygen species and their quantitative determination in biological systems.
我们测试了常用化学发光抑制剂对黄嘌呤氧化酶/次黄嘌呤系统引发的鲁米诺化学发光实例的影响，并试图评估它们在发现导致化学发光的反应途径方面的能力。鲁米诺本身是一种黄嘌呤氧化酶抑制剂，其浓度会影响反应机制。在抑制尿酸生成的鲁米诺浓度下观察到最大化学发光响应。超氧化物歧化酶完全抑制了化学发光，过氧化氢酶的抑制作用取决于鲁米诺浓度。超氧化物的检测剂铁细胞色素c以浓度依赖的方式刺激或抑制化学发光。过氧化物酶强烈刺激化学发光。螯合剂（1 mM去铁胺和0.01 mM二乙基二硫代氨基甲酸盐）对化学发光有明显抑制作用。有人认为，测量鲁米诺化学发光不是区分生物系统中单个活性氧物种及其定量测定的合适方法。
