Effect of Lemon (, L.) Peel Powder on Oocyst Shedding, Intestinal Health, and Performance of Broilers Exposed to Challenge.

To date, no study has reported the anticoccidial effect of lemon peel powder in broilers. Coccidiosis, caused by species, is the prevalent enteric parasitic disease in poultry. Although certain chemical drugs have been used for their control, concerns regarding drug residues and the development of resistance in chickens have arisen among consumers. In this study, a total of 300 Ross 308 broiler chicks were randomly allocated into five groups (five equal replicates of 12 animals). The first group served as the control and did not receive any specific treatment (NC). The second group, referred to as the positive control (PC) group, was deliberately exposed to . The third group was challenged with and also received treatment with amprolium (1 g/kg) and was designated as AT. The fourth and fifth groups were challenged with and simultaneously supplemented with lemon peel powder at a dosage of 3 g/kg (LPP3) and 6 g/kg (LPP6). Sporulated oocysts (5 × 10/mL bird) on day 22 of the experiment were administered to the infected broiler chickens. The results indicated that in comparison with the NC, all -treated birds exhibited significantly ( < 0.05) lower growth performance. However, a notable improvement was observed when infected birds also received a supplement of LPP3 and LPP6 in their feed. Both LPP3 and LPP6 supplementation significantly ( < 0.05) reduced mortality, lesion scores, and oocyst per gram (OPG) of feces compared with the PC group. Additionally, the histological features of the cecum revealed that villus height, villus width, and crypt depth were partially restored under supplementation with LPP3 and LPP6 in the infected birds. Overall, the results demonstrate that -infected birds supplemented with LPP3 and LPP6 exhibited improved growth performance, reduced OPG, lowered intestinal coccidiosis lesion scores, and enhanced intestinal histological features.