Böcking A, Chatelain R, Auffermann W, Krüger G R, Asmus B, Wohltmann D, Schuster C
Anticancer Res. 1986 Sep-Oct;6(5):1205-16.
The prognostic significance of the DNA Malignancy Grade (DNA-MG) was tested for 83 malignant non Hodgkin lymphoma patients in comparison with three different subjective morphological classification systems (New Working Formulation for Clinical Usage, Rappaport and Kiel Classification). Monolayer smears prepared from paraffin embedded tissue and imprint smears from fresh cut lymph-nodes were investigated. Feulgen staining was performed automatically. The scalar DNA-MG was determined by rapid interactive DNA-cytometry, using an automatic microscope and a TV-image analysis system. A strong influence of the DNA-MG on length of survival was found. Compared with morphological classification systems, the DNA-MG was of greater prognostic validity as revealed by different statistical tests. Significant differences of survival probabilities between some groups with differences of 0.5 DNA-MG only were found. The interobserver reproducibility of this new prognostic index was found to be 95% within a range of +/-0.4 DNA-MG.
对83例恶性非霍奇金淋巴瘤患者进行了DNA恶性程度分级（DNA-MG）的预后意义测试，并与三种不同的主观形态学分类系统（临床应用新工作方案、Rappaport分类和Kiel分类）进行比较。研究了从石蜡包埋组织制备的单层涂片和新鲜切除淋巴结的印片。自动进行Feulgen染色。使用自动显微镜和电视图像分析系统，通过快速交互式DNA细胞计数法确定标量DNA-MG。发现DNA-MG对生存时间有强烈影响。与形态学分类系统相比，不同的统计测试表明DNA-MG具有更高的预后有效性。仅在DNA-MG相差0.5的一些组之间发现了生存概率的显著差异。发现该新预后指标在±0.4 DNA-MG范围内的观察者间再现性为95%。
