Guyot Valentin, Ly Ngoc-Sam, Trieu Tien-Dung, Insisiengmay Oudomphone, Zhang Ting, Iskra-Caruana Marie-Line, Pooggin Mikhail M
PHIM Plant Health Institute, University of Montpellier, INRAE, CIRAD, IRD, Institute Agro, 34398 Montpellier, France.
Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City 721400, Vietnam.
Pathogens. 2023 Oct 28;12(11):1289. doi: 10.3390/pathogens12111289.
Banana bunchy top virus is a multicomponent circular ssDNA virus (family ) that causes one of the most devastating diseases of cultivated bananas and plantains (family Musaceae). It is transmitted by the aphids and among host plants of Musaceae and some other families of monocots. Our Illumina sequencing reconstruction of virome components of BBTV-infected banana plants and their neighbor non-banana plants sampled in Vietnam and Laos revealed the monocot sp. (Commelinaceae) and the dicots and (both Asteraceae) as hosts of BBTV and circular ssDNA alphasatellites (family ). Counting the proportions and relative abundances of Illumina reads representing BBTV genome components and alphasatellites suggested that Chromolaena and Commelina are poor hosts for BBTV and one to three alphasatellite species, whereas Bidens is a permissive host for BBTV and four alphasatellite species representing two genera of . Our findings provide evidence for the dicot plants of family Asteraceae as alternative hosts of BBTV and its alphasatellites, which warrants further investigation of these and other dicots as a potential refuge and source of BBTV and multiple alphasatellites that become associated with this virus and likely affect its replication, transmission, and host range.
香蕉束顶病毒是一种多分体环状单链DNA病毒（科），可引发栽培香蕉和大蕉（芭蕉科）最具毁灭性的病害之一。它通过蚜虫在芭蕉科及其他一些单子叶植物科的寄主植物间传播。我们对在越南和老挝采集的感染香蕉束顶病毒的香蕉植株及其相邻非香蕉植株的病毒组成分进行了Illumina测序重建，结果显示单子叶植物鸭跖草属（鸭跖草科）以及双子叶植物胜红蓟属和鬼针草属（均为菊科）是香蕉束顶病毒和环状单链DNAα卫星（科）的寄主。计算代表香蕉束顶病毒基因组成分和α卫星的Illumina读数的比例和相对丰度表明，胜红蓟和鸭跖草是香蕉束顶病毒及一至三种α卫星物种的不良寄主，而鬼针草是香蕉束顶病毒及代表α卫星两个属的四种α卫星物种的适宜寄主。我们的研究结果为菊科双子叶植物作为香蕉束顶病毒及其α卫星的替代寄主提供了证据，这值得对这些及其他双子叶植物作为香蕉束顶病毒和多种α卫星的潜在避难所和来源进行进一步研究，这些α卫星与该病毒相关联，可能会影响其复制、传播和寄主范围。
