Department of Gastroenterology and Internal Medicine, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland.
Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology, Medical University of Warsaw, 02-097 Warsaw, Poland.
Medicina (Kaunas). 2023 Nov 3;59(11):1945. doi: 10.3390/medicina59111945.
Diverticulosis is frequently accompanied by altered bowel habits. The biogenic amines within colonic mucosa control bowel motility, and in particular, alterations in serotonin signaling may play a role in colon diverticulosis. The aim of the study was to assess the concentration of biogenic amines and serotonin receptor expression in the colonic mucosa in patients with diverticulosis and healthy controls. This prospective, comparative study included 59 individuals: 35 with sigmoid diverticulosis and 24 healthy controls. The study was held at the Department of Gastroenterology and Internal Medicine, Medical University of Warsaw, Poland. Mucosal samples were taken from the right and left colon during a colonoscopy in all patients. Concentrations of norepinephrine, 3-methoxy-4-hydroxyphenylglycol, dopamine, homovanillic acid, serotonin, and 5hydroxyindoleacetic acid were measured with high-performance liquid chromatography. Expressions of human 5-hydroxytryptamine receptor 3A, 5-hydroxytryptamine receptor 4, 5-hydroxytryptamine receptor 7, solute carrier family 6 member 4 (SERT) for serotonin, as well as the neuroglia activation markers glial fibrillary acidic protein, S100 calcium-binding protein B, and proteolipid protein 1, were assessed with polymerase chain reaction. The median age and sex distribution were comparable in both study groups (median 69 y vs. 52 y; < 0.455 and males/females in cases 11/17 vs. 18/19 in controls; < 0.309). In diverticulosis patients, there was a higher concentration of serotonin in the left affected colon compared to the right healthy part of the colon (median 8239 pg/mg vs. 6326 pg/mL; < 0.01). The expression was lower in the affected left segment compared to the right colon (median 0.88 vs. 1.36; < 0.01). There was a higher colonic mucosa concentration of serotonin (median 8239 pg/mg vs. 6000 pg/mL; < 0.02) and 5hydroxyindoleacetic acid/serotonin ratio (median 0.27 vs. 0.47; < 0.01) in diverticulosis patients compared to controls in the left side of the colon. The concentration of serotonin in the mucosa of the colon segment affected by diverticula is higher than in the healthy segment in the same individuals and higher than in healthy controls. These results underline serotonin signaling in colon diverticulosis pathophysiology.
憩室病常伴有肠道习惯改变。结肠黏膜中的生物胺控制肠道蠕动，特别是 5-羟色胺信号的改变可能在结肠憩室病中起作用。本研究的目的是评估生物胺浓度和 5-羟色胺受体在憩室病患者和健康对照者结肠黏膜中的表达。
这项前瞻性、对照研究包括 59 名患者：35 名乙状结肠憩室病患者和 24 名健康对照者。研究在波兰华沙医科大学胃肠病学和内科系进行。所有患者在结肠镜检查时均从右结肠和左结肠取黏膜样本。采用高效液相色谱法测定去甲肾上腺素、3-甲氧基-4-羟苯乙二醇、多巴胺、高香草酸、5-羟色胺和 5-羟吲哚乙酸的浓度。采用聚合酶链反应检测人 5-羟色胺受体 3A、5-羟色胺受体 4、5-羟色胺受体 7、5-羟色胺转运体（SERT）以及神经胶质激活标志物胶质纤维酸性蛋白、S100 钙结合蛋白 B 和蛋白脂质蛋白 1 的表达。
两组患者的中位年龄和性别分布相似（中位年龄 69 岁比 52 岁；<0.455 和病例组男性/女性 11/17 比对照组 18/19；<0.309）。在憩室病患者中，左病变结肠的 5-羟色胺浓度高于右健康结肠（中位数 8239 pg/mg 比 6326 pg/mL；<0.01）。病变左侧节段的表达低于右侧结肠（中位数 0.88 比 1.36；<0.01）。与对照组相比，憩室病患者左侧结肠的肠黏膜 5-羟色胺浓度（中位数 8239 pg/mg 比 6000 pg/mL；<0.02）和 5-羟吲哚乙酸/5-羟色胺比值（中位数 0.27 比 0.47；<0.01）更高。
在同一患者中，受憩室影响的结肠节段的黏膜中 5-羟色胺的浓度高于健康节段，且高于健康对照者。这些结果强调了 5-羟色胺信号在结肠憩室病发病机制中的作用。
