Whole-Genome Analysis of Termite-Derived BV-10 and Its Application in King Grass Silage.

(. ) is a cellulose-degrading strain that has the potential as an additive in fermented feed. . BV-10 was isolated and screened from the termite gut. We sequenced the whole genome of this new source of . to reveal its potential for use in cellulose degradation. Whole-genome sequencing of BV-10 showed that it has a circular chromosome of 3929792 bp containing 3873 coding genes with a GC content of 45.51% and many genes related to cellulose, hemicellulose, and lignin degradation. King grass silage was inoculated with BV-10 and mixed with other feed additives to assess the effect of BV-10 on the fermentation quality of silage. Six treatment groups were established: the control, BV-10, molasses, cellulase, BV-10 plus molasses, and BV-10 plus cellulase groups. After 30 days of silage-fermentation testing, BV-10 was found to rapidly reduce the silage pH value and significantly reduce the acid-detergent fiber (ADF) content ( < 0.05). The addition of BV-10 plus molasses and cellulase in fermented feed significantly reduced the silage neutral-detergent fiber and ADF content and promoted organic-acid accumulation ( < 0.05). The above results demonstrate that BV-10 promotes the fermentation quality of silage and that this effect is greater when other silage-fermentation additives are included. In conclusion, genes involved in cellulose degradation in BV-10 were identified by whole-genome sequencing and further experiments explored the effects of BV-10 and different feed additives on the fermentation quality of king grass silage, revealing the potential of as a new silage additive.