Sun Junfen, Pang Hao, Chen Long
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, China.
Materials (Basel). 2023 Nov 17;16(22):7210. doi: 10.3390/ma16227210.
This work reports new mixed matrix membranes (MMMs) for the adsorption of enzymes from organic solvents. In this work, polyimide/hydroxyapatite (PI/HAP) MMMs were prepared via phase inversion method and further crosslinked with 3-aminopropyl triethoxysilane (APTES). The chemical and structural stability of the crosslinked PI/HAP MMMs were improved and applied for lysozyme (LZ) adsorption in organic solvent. PI/HAP MMMs were crosslinked by changing the 3-aminopropyltriethoxysilane (APTES) concentration and crosslinking time. The optimal APTES crosslinking condition for PI/HAP MMMs is 6% of concentration for 8 h. The LZ adsorption performance was studied by changing solvent types. PI/HAP MMMs possessed a high LZ adsorption in organic-solvent-aqueous solutions, and the LZ adsorption capacity reached 34.1 mg/g. The MMMs had a high desorption capacity and recovery ability. The MMMs maintained 60% of their adsorption capacity and 58% of their desorption at the fourth cycle of adsorption and desorption. The MMMs provided a new technology for the purification and separation of enzymes or proteins by MMMs in organic solvents.
这项工作报道了用于从有机溶剂中吸附酶的新型混合基质膜（MMM）。在这项工作中，通过相转化法制备了聚酰亚胺/羟基磷灰石（PI/HAP）MMM，并进一步用3-氨丙基三乙氧基硅烷（APTES）进行交联。交联后的PI/HAP MMM的化学和结构稳定性得到改善，并应用于有机溶剂中溶菌酶（LZ）的吸附。通过改变3-氨丙基三乙氧基硅烷（APTES）的浓度和交联时间对PI/HAP MMM进行交联。PI/HAP MMM的最佳APTES交联条件是浓度为6%，交联时间为8小时。通过改变溶剂类型研究了LZ的吸附性能。PI/HAP MMM在有机溶剂-水溶液中具有较高的LZ吸附量，LZ吸附容量达到34.1 mg/g。该MMM具有较高的解吸能力和回收能力。在吸附和解吸的第四个循环中，MMM保持了其吸附容量的60%和解吸率的58%。该MMM为在有机溶剂中通过MMM纯化和分离酶或蛋白质提供了一种新技术。
