Department of Chemistry, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland.
Department of Molecular Biotechnology and Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Molecules. 2023 Nov 7;28(22):7459. doi: 10.3390/molecules28227459.
The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant ( L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3). Both the extract and the fractions were analyzed in terms of antiradical activity (DPPH and ABTS), total phenolic compounds, and total flavonoids. The antitumor potential of the fractions was evaluated in vitro on human colorectal (HCT 116) and prostate (PC-3) cancer cells. Phenolics were identified using HPLC-QTOF-MS, and twelve compounds were quantified by HPLC-DAD. Finally, principal component analysis was carried out to assess the relationship between the tested factors. The results confirmed that blackcurrant leaves are a rich source of phenolics with high antioxidant activity and anticancer properties. It was demonstrated that the F2 fraction had the highest content of phenolics and the highest antiradical activity. Additionally, only this fraction showed cytotoxic activity against HCT 116 cells. It was confirmed that both the blackcurrant leaf extract and its fractions are a promising source of condensed active compounds and can be used as natural functional food additives.
本研究旨在确定黑加仑（L.）叶及其提取物和化学成分的抗氧化和抗癌特性之间的关系。将干燥的醇提物通过固相萃取分为三个部分：水相（F1）、40%甲醇（F2）和 70%甲醇（F3）。提取物和各部分均根据自由基清除活性（DPPH 和 ABTS）、总酚类化合物和总类黄酮进行分析。通过体外实验评估各部分对人结肠直肠（HCT 116）和前列腺（PC-3）癌细胞的抗肿瘤潜力。采用 HPLC-QTOF-MS 鉴定酚类化合物，并用 HPLC-DAD 定量十二种化合物。最后，进行主成分分析以评估测试因素之间的关系。结果证实，黑加仑叶是一种富含酚类化合物、具有高抗氧化活性和抗癌特性的丰富来源。结果表明，F2 部分的酚类化合物含量最高，自由基清除活性最强。此外，只有该部分对 HCT 116 细胞表现出细胞毒性活性。证实黑加仑叶提取物及其各部分是浓缩活性化合物的有前途的来源，可作为天然功能性食品添加剂使用。
