Department of Chemistry, Payame Noor University (PNU), Mashhad, Iran; Department of Chemistry, Payame Noor University, P.O. Box 19395-4697, Tehran 19569, Iran.
Department of Chemistry, Payame Noor University (PNU), Mashhad, Iran.
Mater Sci Eng C Mater Biol Appl. 2018 Apr 1;85:225-232. doi: 10.1016/j.msec.2017.05.099. Epub 2017 May 17.
Preparation of a molecularly imprinted polymer (MIP) film and its recognition property for methimazole (MMZ) was investigated. The polypyrrole (PPy) film was prepared by the cyclic voltammetric deposition of pyrrole in the presence of a supporting electrolyte (NaClO·HO) with and without MMZ through on a pencil graphite electrode (PGE). A computational study based on density functional theory was developed to evaluate the template-monomer geometry and interaction energy in the prepolymerization mixture. The performance of MIP sensor and non-imprinted polymer (NIP) film was evaluated by differential pulse voltammetry (DPV). The most important parameters controlling the performance of sensor were investigated and optimized. The prepared electrode was used for MMZ measurement by a three-step procedure, including analyte extraction in the electrode, electrode washing and electrochemical measurement of MMZ. The molecularly imprinted film exhibited a high selectivity and sensitivity toward methimazole in the experimental conditions. The calibration curve demonstrated linearity over a concentration range of 0.007-6mM with a correlation coefficient (r) of 0.9808. The accuracy of the method was studied through spiking blank samples showed recovery of 98% with precision of 4%. Limit of detection based on S/N=3 was obtained 3×10M. The proposed sensor was applied successfully to determine MMZ in biological model samples and pharmaceuticals.
研究了一种用于甲巯咪唑（MMZ）的分子印迹聚合物（MIP）薄膜的制备及其识别性能。通过在含有和不含有 MMZ 的支持电解质（NaClO·HO）存在下，在铅笔石墨电极（PGE）上通过循环伏安沉积吡咯，制备了聚吡咯（PPy）薄膜。基于密度泛函理论的计算研究用于评估预聚合混合物中模板-单体的几何形状和相互作用能。通过差分脉冲伏安法（DPV）评估 MIP 传感器和非印迹聚合物（NIP）薄膜的性能。研究和优化了控制传感器性能的最重要参数。通过包括在电极中提取分析物、电极洗涤和电化学测量 MMZ 的三步程序，使用制备的电极进行 MMZ 测量。在实验条件下，分子印迹膜对甲巯咪唑表现出高选择性和灵敏度。校准曲线在 0.007-6mM 的浓度范围内表现出线性关系，相关系数（r）为 0.9808。通过向空白样品中添加 MMZ 来研究该方法的准确性，回收率为 98%，精密度为 4%。基于 S/N=3 的检测限为 3×10M。该传感器成功应用于生物模型样品和药物中 MMZ 的测定。
