Department of Biology and Biotechnology, American University of Madaba, Madaba 11821, Jordan.
Faculty of Data Science and Information Technology, INTI International University, Nilai 71800, Malaysia.
Molecules. 2023 Nov 9;28(22):7502. doi: 10.3390/molecules28227502.
Exposure to water-pipe smoking, whether flavored or unflavored, has been shown to instigate inflammation and oxidative stress in BALB/c mice. This consequently results in alterations in the expression of inflammatory markers and antioxidant genes. This study aimed to scrutinize the impact of Epigallocatechin gallate (EGCG)-a key active component of green tea-on inflammation and oxidative stress in BALB/c mice exposed to water-pipe smoke. The experimental setup included a control group, a flavored water-pipe smoke (FWP) group, an unflavored water-pipe smoke (UFWP) group, and EGCG-treated flavored and unflavored groups (FWP + EGCG and UFWP + EGCG). Expression levels of IL-6, IL1B, TNF-α, CAT, GPXI, MT-I, MT-II, SOD-I, SOD-II, and SOD-III were evaluated in lung, liver, and kidney tissues. Histopathological changes were also assessed. The findings revealed that the EGCG-treated groups manifested a significant decline in the expression of inflammatory markers and antioxidant genes compared to the FWP and UFWP groups. This insinuates that EGCG holds the capacity to alleviate the damaging effects of water-pipe smoke-induced inflammation and oxidative stress. Moreover, enhancements in histopathological features were observed in the EGCG-treated groups, signifying a protective effect against tissue damage induced by water-pipe smoking. These results underscore the potential of EGCG as a protective agent against the adverse effects of water-pipe smoking. By curbing inflammation and oxidative stress, EGCG may aid in the prevention or mitigation of smoking-associated diseases.
水烟吸烟，无论是有味道的还是无味道的，已被证明会在 BALB/c 小鼠中引发炎症和氧化应激。这会导致炎症标志物和抗氧化基因表达的改变。本研究旨在探究表没食子儿茶素没食子酸酯（EGCG）——绿茶的一种主要活性成分——对水烟烟雾暴露的 BALB/c 小鼠炎症和氧化应激的影响。实验设置包括对照组、有味道水烟（FWP）组、无味道水烟（UFWP）组以及 EGCG 处理的有味道和无味道组（FWP + EGCG 和 UFWP + EGCG）。评估了 IL-6、IL1B、TNF-α、CAT、GPXI、MT-I、MT-II、SOD-I、SOD-II 和 SOD-III 在肺、肝和肾组织中的表达水平。还评估了组织病理学变化。研究结果表明，与 FWP 和 UFWP 组相比，EGCG 处理组的炎症标志物和抗氧化基因表达显著下降。这表明 EGCG 具有减轻水烟烟雾引起的炎症和氧化应激的破坏性影响的能力。此外，在 EGCG 处理组中观察到组织病理学特征的改善，表明对水烟吸烟引起的组织损伤具有保护作用。这些结果强调了 EGCG 作为一种对抗水烟吸烟不良影响的保护剂的潜力。通过抑制炎症和氧化应激，EGCG 可能有助于预防或减轻与吸烟相关的疾病。
