State Key Laboratory of Chemistry and Utilization of Carbon-Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi 830017, China.
Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chemical Engineering, Yili Normal University, Yining 835000, China.
Sensors (Basel). 2023 Nov 15;23(22):9187. doi: 10.3390/s23229187.
Selenium (Se), as one of the essential and nutrient components of living organisms and plants, plays an important role in life activities, while excessive selenium is hazardous to human health. So, the establishment of an effective method for simple, rapid, and highly sensitive determination of selenium content is crucial in the field of food composition analysis and other areas. In this paper, a novel and simple ratiometric fluorescence method for the determination of Se has been developed using 9-anthracenemethanol (AM) as the ratiometric fluorescence reagent on the basis of the conventional fluorometric assay which utilized 2,3-diaminonapthalene (DAN) as fluorescent ligand. The ratiometric method was compared with the conventional method with respect to precision and accuracy. The inter-day and intra-day precisions (RSDs) of the ratiometric fluorescence method ranged from 2.08 to 2.78% and 1.28 to 1.84%, with mean recoveries of 93.2~98.0% and limit of detection (LOD) and limit of quantification (LOQ) of 0.0016 and 0.0049 μg/mL, respectively. This method was successfully applied to the determination of total selenium in selenium-enriched milk and selenium-supplemented shampoo, with the results in agreement with those obtained by inductively coupled plasma mass spectrometer (ICP-MS). The results demonstrated that the precision and accuracy of the ratiometric fluorescence method were superior to those of the conventional fluorescence method, and the interferences of various environmental factors were effectively eliminated. The precision and accuracy of the conventional method can be significantly improved by simply adding an elaborately selected ratiometric fluorescence reagent, and the new method will have broader practical applications.
硒（Se）是生物和植物必需的营养元素之一，在生命活动中发挥着重要作用，而过量的硒对人体健康有害。因此，建立一种简单、快速、高灵敏度的硒含量测定方法对于食品成分分析等领域至关重要。本文在传统荧光法的基础上，以 9-蒽甲醇（AM）为比率荧光试剂，开发了一种新颖、简单的比率荧光法测定硒。该方法在精密度和准确度方面与传统方法进行了比较。比率荧光法的日内和日间精密度（RSD）分别为 2.08%2.78%和 1.28%1.84%，平均回收率为 93.2%~98.0%，检测限（LOD）和定量限（LOQ）分别为 0.0016 和 0.0049μg/mL。该方法成功应用于富硒牛奶和硒补充洗发水总硒的测定，与电感耦合等离子体质谱（ICP-MS）法的结果一致。结果表明，比率荧光法的精密度和准确度优于传统荧光法，有效消除了各种环境因素的干扰。通过简单地添加精心选择的比率荧光试剂，可显著提高传统方法的精密度和准确度，新方法将具有更广泛的实际应用。
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