Key Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security, Beijing 100193, China.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Fa Yi Xue Za Zhi. 2023 Oct 25;39(5):457-464. doi: 10.12116/j.issn.1004-5619.2022.320101.
To establish the GC-MS qualitative and quantitative analysis methods for the synthetic cannabinoids, its main matrix and additives in suspicious electronic cigarette (e-cigarette) oil samples.
The e-cigarette oil samples were analyzed by GC-MS after diluted with methanol. Synthetic cannabinoids, its main matrix and additives in e-cigarette oil samples were qualitatively analyzed by the characteristic fragment ions and retention time. The synthetic cannabinoids were quantitatively analyzed by using the selective ion monitoring mode.
The linear range of each compound in GC-MS quantitative method was 0.025-1 mg/mL, the matrix recovery rate was 94%-103%, the intra-day precision relative standard deviations (RSD) was less than 2.5%, and inter-day precision RSD was less than 4.0%. Five indoles or indazole amide synthetic cannabinoids were detected in 25 e-cigarette samples. The main matrixes of e-cigarette samples were propylene glycol and glycerol. Additives such as ,2,3-trimethyl-2-isopropyl butanamide (WS-23), glycerol triacetate and nicotine were detected in some samples. The content range of synthetic cannabinoids in 25 e-cigarette samples was 0.05%-2.74%.
The GC-MS method for synthesizing cannabinoid, matrix and additive in e-cigarette oil samples has good selectivity, high resolution, low detection limit, and can be used for simultaneous qualitative and quantitative analysis of multiple components; The explored fragment ion fragmentation mechanism of the electron bombardment ion source of indole or indoxamide compounds helps to identify such substances or other compounds with similar structures in cases.
建立电子卷烟（电子烟）油样品中合成大麻素及其主要基质和添加剂的 GC-MS 定性和定量分析方法。
将电子烟油样品用甲醇稀释后，用 GC-MS 进行分析。通过特征碎片离子和保留时间对电子烟油样品中的合成大麻素及其主要基质和添加剂进行定性分析。采用选择离子监测模式对合成大麻素进行定量分析。
GC-MS 定量方法中各化合物的线性范围为 0.025-1mg/mL，基质回收率为 94%-103%，日内精密度相对标准偏差（RSD）小于 2.5%，日间精密度 RSD 小于 4.0%。在 25 份电子烟样品中检测到 5 种吲哚或吲哚酰胺类合成大麻素。电子烟样品的主要基质为丙二醇和甘油。在一些样品中检测到添加剂，如 WS-23（2,3-二甲基-2-异丙基丁酰胺）、甘油三乙酸酯和尼古丁。25 份电子烟样品中合成大麻素的含量范围为 0.05%-2.74%。
GC-MS 法用于电子烟油样品中合成大麻素、基质和添加剂的分析，具有良好的选择性、高分辨率、低检测限，可用于多种成分的同时定性定量分析；电子轰击离子源对吲哚或吲哚酰胺类化合物的碎片离子碎裂机制的探索有助于识别此类物质或其他具有类似结构的化合物。
