Centre for Incubation, Innovation, Research and Consultancy, Jyothy Institute of Technology, Bengaluru, India.
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences and Center of Excellence in Bioactive Resources for Innovative Clinical Applications, Chulalongkorn University, Bangkok, Thailand.
J Biomed Mater Res B Appl Biomater. 2024 Jan;112(1):e35343. doi: 10.1002/jbm.b.35343. Epub 2023 Nov 25.
A thin plastic-like film separated from the epidermis of Cereus hildmannianus has excellent tensile strength, resistance to water and high antimicrobial activity and supports the growth of mouse fibroblast cells. Cactuses are one of the most under explored plant species with high potential for food, materials, pharmaceutical and other applications. Although studies have shown the ability of cactuses to be used for food, as a source for fibers, as reinforcement for composites and other applications, the role of individual layers and their properties has been studied to a limited extent. In this paper, a thin translucent layer was separated from the epidermis of C. hildmannianus and studied for its composition, structure and properties. The layer is composed of about 73% cellulose and 2% lignin and morphologically, shows surface with uneven and serrated edges. Films with length of up to 36 cm, strength of 6.8 MPa and elongation of 2.5% could be peeled from the cactus suggesting their suitability for food packaging and other applications. X-ray diffraction patterns and FTIR spectrums indicated that the films are similar to that of cellulose and major thermal degradation occurred above 280°C. Compared to standards, the cactus films showed about 41% and 44% inhibition against gram positive and gram negative bacteria and 67% inhibition of the common fungal strain (A. niger). Films showed high stability in water and to common chemicals. When used as substrates for mouse fibroblast cell growth, no cytotoxicity was observed and the cactus peel supported the attachment and proliferation of cells demonstrating potential to be used as a biomaterial for tissue engineering applications.
从 Cereus hildmannianus 表皮分离出的一种薄的类似塑料的薄膜，具有优异的拉伸强度、耐水性和高抗菌活性，并支持小鼠成纤维细胞的生长。仙人掌是最未被充分探索的植物物种之一，具有很高的食品、材料、制药和其他应用潜力。尽管研究表明仙人掌有能力用作食物、纤维来源、复合材料增强剂等，但对个别层及其特性的研究还很有限。在本文中，从 C. hildmannianus 的表皮分离出一种薄的半透明层，并研究其组成、结构和性能。该层由约 73%的纤维素和 2%的木质素组成，形态上表现出表面不均匀和锯齿状边缘。可以从仙人掌上剥离长达 36cm、强度为 6.8MPa、伸长率为 2.5%的薄膜，表明它们适合用于食品包装和其他应用。X 射线衍射图谱和 FTIR 光谱表明，这些薄膜类似于纤维素，主要热降解发生在 280°C 以上。与标准相比，仙人掌薄膜对革兰氏阳性菌和革兰氏阴性菌的抑制率分别约为 41%和 44%，对常见真菌（黑曲霉）的抑制率为 67%。薄膜在水中和常见化学品中具有高稳定性。用作小鼠成纤维细胞生长的基质时，未观察到细胞毒性，仙人掌皮支持细胞的附着和增殖，表明有潜力用作组织工程应用的生物材料。
