Division of Pharmaceutical and Fine Chemical Technology, Department of Chemical Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, West Bengal, India.
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711103, West Bengal, India.
Int J Biol Macromol. 2021 Jun 1;180:339-354. doi: 10.1016/j.ijbiomac.2021.03.034. Epub 2021 Mar 9.
This work intends to synthesis newer guar gum indole acetate ester and design film scaffolds based on protein-polysaccharide interactions for tissue engineering applications. Guar gum indole acetate(GGIA) was synthesized for the first time from guar gum in presence of aprotic solvent activated hofmeister ions. The newer biopolymer was fully characterized in FT-IR,C NMR, XRD and TGA analysis. High DS (Degree of Substitution, DS = 0.61) GGIA was cross-linked with hydrolyzed keratin, extracted from chicken feather wastes. Films were synthesized from different biopolymer ratios and the surface chemistry appeared interesting. Physicochemical properties for GGIA-keratin association were notable. Fully bio-based films were non-cytotoxic and exhibited excellent biocompatibility for human dermal fibroblast cell cultivations. The film scaffold showed 63% porosity and the recorded tensile strength at break was 6.4 MPa. Furthermore, the standardised film exerted superior antimicrobial activity against both the Gram-positive and Gram-negative bacteria. MICs were recorded at 130 μg/mL and 212 μg/mL for E. coli and S. aureus respectively. In summary, GGIA-keratin film scaffolds represented promising platforms for skin tissue engineering applications.
这项工作旨在合成新型瓜尔胶吲哚乙酸酯，并基于蛋白质-多糖相互作用设计用于组织工程应用的薄膜支架。首次在无质子溶剂激活的霍夫迈斯特离子存在下从瓜尔胶中合成了新型生物聚合物瓜尔胶吲哚乙酸酯（GGIA）。通过 FT-IR、C NMR、XRD 和 TGA 分析对新型生物聚合物进行了全面表征。高取代度（DS=0.61）的 GGIA 与从鸡毛废物中提取的水解角蛋白交联。根据不同生物聚合物的比例合成了薄膜，并观察到有趣的表面化学性质。GGIA-角蛋白结合的物理化学性质值得注意。完全基于生物的薄膜无细胞毒性，并表现出对人真皮成纤维细胞培养的优异生物相容性。该薄膜支架的孔隙率为 63%，记录的断裂拉伸强度为 6.4 MPa。此外，该标准薄膜对革兰氏阳性菌和革兰氏阴性菌均表现出优异的抗菌活性。大肠杆菌和金黄色葡萄球菌的 MIC 分别为 130 μg/mL 和 212 μg/mL。总之，GGIA-角蛋白薄膜支架代表了用于皮肤组织工程应用的有前途的平台。
