CXCL9 和 NT-proBNP：类风湿关节炎中炎症介质和心血管疾病生物标志物之间的显著关联。

CXCL9 and NT-proBNP, a notable link between inflammatory mediator and cardiovascular disease biomarker in rheumatoid arthritis.

机构信息

Immunology Department, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

出版信息

Clin Rheumatol. 2024 Jan;43(1):137-145. doi: 10.1007/s10067-023-06826-y. Epub 2023 Nov 25.

DOI:10.1007/s10067-023-06826-y

PMID:38006573

Abstract

INTRODUCTION

Cardiovascular disease (CVD) is the most critical extra-articular manifestation of rheumatoid arthritis, and inflammatory molecules contribute to its pathogenesis. Recently, CXCL9 has been considered an inflammatory chemokine associated with the pathogenesis of CVD. Here, we evaluated the association of plasma CXCL9 with well-established cardiac biomarkers, including HS-CRP (High sensitivity C-reactive protein) and NT-ProBNP (N-terminal pro-B-type natriuretic peptide), in newly diagnosed and under-treatment RA patients.

METHODS

Thirty newly diagnosed patients, 30 under-treatment RA patients, and 30 healthy subjects were recruited. The plasma concentration of CXCL9 and NT-ProBNP was measured using the ELISA method. The HS-CRP levels was measured in plasma samples using latex-enhanced immunoturbidimetric test.

RESULTS

We found increased plasma levels of CXCL9, HS-CRP, and NT-proBNP in RA patients compared to healthy subjects, besides that the concentration of CXCL9, HS-CRP, and NT-ProBNP showed elevated levels in newly diagnosed RA patients compared to under-treatment group. The mean plasma concentration of CXCL9, NT-proBNP, and HS-CRP were statistically different among healthy subjects, newly diagnosed, and under-treatment RA patients (p < 0.001, p = 0.016, and p < 0.001, respectively). We also found a significant positive correlation between CXCL9 and DAS-28 (p = 0.0005, r = 0.436) in the patients' group (new-case + under-treatment). There was a significantly positive correlation between CXCL9 and NT-proBNP in newly diagnosed and under-treatment patients (p = 0.020, r = 0.424; p < 0.0001, r = 0.853, respectively). In the patient's group (new-case + under-treatment), there was a significantly positive correlation between CXCL9 with NT-proBNP (p < 0.001, r = 0.703) and CXCL9 with HS-CRP (p = 0.015, r = 0.313).

CONCLUSION

CXCL9 correlates significantly with well-established cardiovascular biomarkers, including HS-CRP and NT-ProBNP in RA patients. Key Points • CXCL9 is an inflammatory marker in RA. • CXCL9 has correlated with DAS-28. • There is a strong correlation between CXCL9 with NT-proBNP and HS-CRP.

摘要

简介

心血管疾病（CVD）是类风湿关节炎最重要的关节外表现，炎症分子参与其发病机制。最近，CXCL9 被认为是一种与 CVD 发病机制相关的炎症趋化因子。在这里，我们评估了血浆 CXCL9 与既定的心脏生物标志物（包括高敏 C 反应蛋白（hs-CRP）和 N 末端 pro-B 型利钠肽（NT-ProBNP））在新诊断和治疗中的 RA 患者之间的相关性。

方法

招募了 30 名新诊断的患者、30 名治疗中的 RA 患者和 30 名健康受试者。使用 ELISA 法测量 CXCL9 和 NT-ProBNP 的血浆浓度。使用乳胶增强免疫比浊法测量血浆样品中的 hs-CRP 水平。

结果

我们发现 RA 患者的血浆 CXCL9、hs-CRP 和 NT-proBNP 水平升高，与健康受试者相比，新诊断的 RA 患者的 CXCL9、hs-CRP 和 NT-proBNP 浓度高于治疗中的组。健康受试者、新诊断和治疗中的 RA 患者之间的平均血浆 CXCL9、NT-proBNP 和 hs-CRP 浓度存在统计学差异（p<0.001、p=0.016 和 p<0.001，分别）。我们还发现患者组（新诊断+治疗中）中 CXCL9 与 DAS-28 之间存在显著正相关（p=0.0005，r=0.436）。新诊断和治疗中的患者之间的 CXCL9 与 NT-proBNP 之间存在显著正相关（p=0.020，r=0.424；p<0.0001，r=0.853，分别）。在患者组（新诊断+治疗中）中，CXCL9 与 NT-proBNP（p<0.001，r=0.703）和 CXCL9 与 hs-CRP（p=0.015，r=0.313）之间存在显著正相关。