Shamsi Afsaneh, Roghani Seyed Askar, Shamsi Mohammad, Jalili Cyrus, Taghadosi Mahdi, Soufivand Parviz
Immunology Department, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Heliyon. 2024 Aug 23;10(17):e36763. doi: 10.1016/j.heliyon.2024.e36763. eCollection 2024 Sep 15.
Cardiovascular disease (CVD) is the most important comorbid condition in rheumatoid arthritis (RA) patients. Dysregulated expression of non-coding RNA families has a critical role in RA-associated inflammatory events, including cardiovascular manifestations. The long non-coding RNA (lncRNA)- HIX003209 has a role in RA associated inflammation. In the current study, we investigated the association of HIX003209 and its downstream microRNA, miR-6089, with various cardiovascular and inflammatory biomarkers in RA patients.
60 RA patients, including 30 newly diagnosed and 30 on-treatment patients were recruited in this study, and 30 healthy people were selected as a control group. The gene expression of HIX003209, miR-6089, and CXCR3 were measured using Real-time PCR. The CVD risk was measured using Systematic Coronary Risk Evaluation (SCORE) and Framingham Risk Score (FRS).
The gene expression of LncRNA-HIX003209 was elevated significantly in newly-diagnosed compared to under-treatment and control groups (p < 0.05). The miR-6089 gene expression was elevated significantly in under-treatment RA patients group compared to control group (p < 0.001). There was a significant positive correlation between LncRNA-HIX003209 with CXCR3 gene expression (p < 0.01, r = 0.341). There was a significantly negative correlation between the gene expression of miR-6089 with DAS-28 (p < 0.05, r = -0.309), NT-proBNP plasma level (p = 0.039, r = -0.268), and CXCL9 plasma level (p < 0.001, r = -0.421).
Regarding its anti-inflammatory effects, miR-6089 may play an important role in preventing the pathological events of cardiovascular disorders in RA patients, through its inhibitory effects on inflammatory chemokines, such as CXCL9, and NT-ProBNP. Higher expression of LncRNA-HIX003209 may disrupt the anti-inflammatory effect of miR-6089 in RA patients.
心血管疾病（CVD）是类风湿关节炎（RA）患者中最重要的合并症。非编码RNA家族的表达失调在包括心血管表现在内的RA相关炎症事件中起关键作用。长链非编码RNA（lncRNA）-HIX003209在RA相关炎症中起作用。在本研究中，我们调查了RA患者中HIX003209及其下游微小RNA miR-6089与各种心血管和炎症生物标志物之间的关联。
本研究招募了60例RA患者，包括30例新诊断患者和30例正在接受治疗的患者，并选择30名健康人作为对照组。使用实时PCR测量HIX003209、miR-6089和CXCR3的基因表达。使用系统性冠状动脉风险评估（SCORE）和弗雷明汉风险评分（FRS）测量CVD风险。
与治疗不足组和对照组相比，新诊断组中LncRNA-HIX003209的基因表达显著升高（p < 0.05）。与对照组相比，治疗不足的RA患者组中miR-6089基因表达显著升高（p < 0.001）。LncRNA-HIX003209与CXCR3基因表达之间存在显著正相关（p < 0.01，r = 0.341）。miR-6089的基因表达与DAS-28（p < 0.05，r = -0.309）、NT-proBNP血浆水平（p = 0.039，r = -0.268）和CXCL9血浆水平（p < 0.001，r = -0.421）之间存在显著负相关。
鉴于其抗炎作用，miR-6089可能通过对炎症趋化因子（如CXCL9）和NT-ProBNP的抑制作用，在预防RA患者心血管疾病的病理事件中发挥重要作用。LncRNA-HIX003209的高表达可能会破坏RA患者中miR-6089的抗炎作用。
