多胺对分离肝细胞中苯丙氨酸羟化酶磷酸化状态和酶活性的依赖性调节
The polyamine-dependent modulation of phenylalanine hydroxylase phosphorylation state and enzymic activity in isolated liver cells.
作者信息
Fisher M J, Dickson A J, Pogson C I
出版信息
Biochem J. 1986 Jul 1;237(1):277-9. doi: 10.1042/bj2370277.
Abstract
The role of polyamines in the control of phenylalanine hydroxylase phosphorylation state and enzymic activity was investigated. Pre-treatment of liver cells with spermine (1 mM) abolishes the glucagon (1 nM)-stimulated increase in hydroxylase phosphorylation. Concurrently there is a decrease in phenylalanine hydroxylation flux, reflecting decreased enzyme activity; 50% inhibition occurs at approx. 10 microM-spermine. These results are discussed in the context of reports concerning the properties of protein phosphatase 2A.
摘要
研究了多胺在控制苯丙氨酸羟化酶磷酸化状态和酶活性中的作用。用精胺（1 mM）预处理肝细胞可消除胰高血糖素（1 nM）刺激引起的羟化酶磷酸化增加。同时，苯丙氨酸羟化通量降低，反映出酶活性降低；在约10 microM精胺时出现50%的抑制。在有关蛋白磷酸酶2A特性的报告背景下讨论了这些结果。
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引用本文的文献
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