Damuni Z, Humphreys J S, Reed L J
Biochem Biophys Res Commun. 1984 Oct 15;124(1):95-9. doi: 10.1016/0006-291x(84)90921-5.
Pyruvate dehydrogenase phosphatase requires Mg2+ or Mn2+, and its activity in the presence of Mg2+ is markedly stimulated by Ca2+. At saturating Mg2+ and Ca2+ concentrations, the polyamines spermine, spermidine and putrescine stimulated the activity of pyruvate dehydrogenase phosphatase 1.5- to 3-fold. Spermine was the most active of the polyamines. At a physiological concentration of Mg2+ (1 mM) and saturating Ca2+ concentration, the stimulation by 0.5 mM spermine was 4- to 5-fold, and at 0.3 mM Mg2+, the stimulation was 20- to 30-fold. In the absence of Mg2+ or Ca2+, spermine had no effect. These results suggest that a polybasic factor may be involved in the regulation of pyruvate dehydrogenase phosphatase activity.
丙酮酸脱氢酶磷酸酶需要Mg2+或Mn2+，并且在Mg2+存在的情况下其活性会受到Ca2+的显著刺激。在Mg2+和Ca2+浓度饱和时，多胺精胺、亚精胺和腐胺可使丙酮酸脱氢酶磷酸酶的活性提高1.5至3倍。精胺是多胺中活性最强的。在生理浓度的Mg2+（1 mM）和饱和Ca2+浓度下，0.5 mM精胺的刺激作用为4至5倍，而在0.3 mM Mg2+时，刺激作用为20至30倍。在没有Mg2+或Ca2+的情况下，精胺没有作用。这些结果表明，一种多碱性因子可能参与了丙酮酸脱氢酶磷酸酶活性的调节。
