Dakshinamurti K, Bhullar R P, Scoot A, Rector E S, Delespesse G, Sehon A H
Biochem J. 1986 Jul 15;237(2):477-82. doi: 10.1042/bj2370477.
Monoclonal antibodies to biotin have been prepared by using biotin linked to keyhole limpet haemocyanin (KLH) as the antigen. Spleen cells obtained from mice immunized with biotin-KLH were fused with the myeloma cell line NS-1. The resulting hybridomas were screened for the production of antibodies to biotin using an enzyme-linked immunosorbent assay. Clones producing antibodies to biotin were isolated by limiting dilution methods. Four cell lines, each derived originally from a different fusion, were chosen for the production of monoclonal antibodies. The monoclonal antibodies obtained have been characterized with respect to their ability to interact with biotin, biotin-bovine serum albumin, biotin-KLH and biocytin as well as to inhibit biotin-dependent enzymes. They have been used to produce cellular biotin deficiency in vitro for studies of biotin function.
通过使用与钥孔戚血蓝蛋白（KLH）相连的生物素作为抗原，制备了抗生物素单克隆抗体。从用生物素-KLH免疫的小鼠中获得的脾细胞与骨髓瘤细胞系NS-1融合。使用酶联免疫吸附测定法筛选所得杂交瘤产生抗生物素抗体的情况。通过有限稀释法分离产生抗生物素抗体的克隆。选择四个最初源自不同融合的细胞系来生产单克隆抗体。所获得的单克隆抗体已就其与生物素、生物素-牛血清白蛋白、生物素-KLH和生物胞素相互作用的能力以及抑制生物素依赖性酶的能力进行了表征。它们已被用于在体外产生细胞生物素缺乏，以研究生物素的功能。
