[制备抗甲状腺素单克隆抗体]
[Preparing monoclonal antibodies to thyroxine].
作者信息
Danilova N D, Krupina N V, Mertts M V, Vasilov R G
出版信息
Bioorg Khim. 1993 Jul;19(7):704-12.
Spleen cells of BALB/c mouse immunized with the keyhole limpet haemocyanin and thyroxine conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells to produce. Four monoclonal antibodies selected by indirect ELISA and partially characterized. One antibody, 1B7, chosen for immunoassay, was produced in mouse ascites fluid, purified and analyzed; it proved to belong to the IgG1 subclass. The cross-reactivity with triiodothyronine was less than 1%, the association constant was 3 x 10(9) M-1.
用钥孔戚血蓝蛋白与甲状腺素偶联物免疫的BALB/c小鼠的脾细胞与P3-X63-Ag8.653小鼠骨髓瘤细胞融合以产生。通过间接ELISA选择了四种单克隆抗体并进行了部分特性鉴定。选择一种抗体1B7用于免疫测定，它在小鼠腹水中产生，经过纯化和分析；结果证明它属于IgG1亚类。与三碘甲状腺原氨酸的交叉反应率小于1%，缔合常数为3×10⁹ M⁻¹。
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