Wilkinson T C, Wilton D C
Biochem J. 1986 Sep 1;238(2):419-24. doi: 10.1042/bj2380419.
Fatty acid-binding protein from rat liver is shown to bind the fluorescent fatty acid probe dansyl undecanoic acid. Binding is accompanied by a shift in the fluorescence emission maximum from 550 nm to 500 nm and a 60-fold fluorescence enhancement at 500 nm. These spectral properties have allowed the use of this probe to detect and quantify microgram amounts of liver fatty acid-binding protein during purification procedures. In conjunction with h.p.l.c. the method allows the rapid estimation of liver fatty acid-binding protein in biological samples. The validity of the method is demonstrated by measuring the concentration of fatty acid-binding protein in livers from control and hypolipidaemic-drug-treated rats. The dramatic diurnal rhythm previously reported for this protein [Dempsey (1984) Curr. Top. Cell. Regul. 24, 63-86] was not observed with this method.
已证明大鼠肝脏中的脂肪酸结合蛋白能结合荧光脂肪酸探针丹磺酰十一烷酸。结合过程伴随着荧光发射最大值从550nm移至500nm，且在500nm处荧光增强60倍。这些光谱特性使得该探针可用于在纯化过程中检测和定量微克级的肝脏脂肪酸结合蛋白。结合高效液相色谱法，该方法可快速估算生物样品中的肝脏脂肪酸结合蛋白。通过测量对照大鼠和经降血脂药物处理的大鼠肝脏中脂肪酸结合蛋白的浓度，证明了该方法的有效性。用该方法未观察到先前报道的这种蛋白显著的昼夜节律[邓普西（1984年）《细胞调节当前专题》24，63 - 86]。
