脂肪酸结合蛋白的研究。大鼠肝脏脂肪酸结合蛋白的结合特性。
Studies on fatty acid-binding proteins. The binding properties of rat liver fatty acid-binding protein.
作者信息
Wilkinson T C, Wilton D C
机构信息
Department of Biochemistry, University of Southampton, U.K.
出版信息
Biochem J. 1987 Oct 15;247(2):485-8. doi: 10.1042/bj2470485.
Abstract
- The fluorescent fatty acid probe 11-(dansylamino)undecanoic acid binds to rat liver fatty acid-binding protein with a 1:1 stoichiometry. 2. The binding of the fluorescent probe is competitive with long-chain fatty acids. 3. Binding displacement studies were performed with a wide range of fatty acids and other ligands and identified C16 and C18 fatty acids as the preferred fatty acids for rat liver fatty acid-binding protein. No preference was observed for unsaturated fatty acids within this group. 4. Fatty acyl-CoA binds less well than the corresponding fatty acid.
摘要
- 荧光脂肪酸探针11-（丹磺酰氨基）十一烷酸以1:1的化学计量比与大鼠肝脏脂肪酸结合蛋白结合。2. 荧光探针的结合与长链脂肪酸具有竞争性。3. 用多种脂肪酸和其他配体进行了结合置换研究，确定C16和C18脂肪酸是大鼠肝脏脂肪酸结合蛋白的优选脂肪酸。在该组内未观察到对不饱和脂肪酸的偏好。4. 脂肪酰辅酶A的结合不如相应脂肪酸好。
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