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脂肪酸结合蛋白的研究。大鼠肝脏脂肪酸结合蛋白的结合特性。

Studies on fatty acid-binding proteins. The binding properties of rat liver fatty acid-binding protein.

作者信息

Wilkinson T C, Wilton D C

机构信息

Department of Biochemistry, University of Southampton, U.K.

出版信息

Biochem J. 1987 Oct 15;247(2):485-8. doi: 10.1042/bj2470485.

DOI:10.1042/bj2470485
PMID:3426548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148433/
Abstract
  1. The fluorescent fatty acid probe 11-(dansylamino)undecanoic acid binds to rat liver fatty acid-binding protein with a 1:1 stoichiometry. 2. The binding of the fluorescent probe is competitive with long-chain fatty acids. 3. Binding displacement studies were performed with a wide range of fatty acids and other ligands and identified C16 and C18 fatty acids as the preferred fatty acids for rat liver fatty acid-binding protein. No preference was observed for unsaturated fatty acids within this group. 4. Fatty acyl-CoA binds less well than the corresponding fatty acid.
摘要
  1. 荧光脂肪酸探针11-（丹磺酰氨基）十一烷酸以1:1的化学计量比与大鼠肝脏脂肪酸结合蛋白结合。2. 荧光探针的结合与长链脂肪酸具有竞争性。3. 用多种脂肪酸和其他配体进行了结合置换研究，确定C16和C18脂肪酸是大鼠肝脏脂肪酸结合蛋白的优选脂肪酸。在该组内未观察到对不饱和脂肪酸的偏好。4. 脂肪酰辅酶A的结合不如相应脂肪酸好。

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本文引用的文献

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Fatty-acid-binding proteins. Occurrence of two fatty-acid-binding proteins in bovine liver cytosol and their binding of fatty acids, cholesterol, and other lipophilic ligands.脂肪酸结合蛋白。牛肝细胞溶质中两种脂肪酸结合蛋白的存在及其与脂肪酸、胆固醇和其他亲脂性配体的结合。
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Spectroscopic investigations on the binding site of bovine hepatic fatty acid binding protein. Evidence for the existence of a single binding site for two fatty acid molecules.牛肝脏脂肪酸结合蛋白结合位点的光谱学研究。存在两个脂肪酸分子的单一结合位点的证据。
Chem Phys Lipids. 1985 Aug 30;38(1-2):159-73. doi: 10.1016/0009-3084(85)90064-7.
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J Biol Chem. 1985 Nov 25;260(27):14521-8.
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