Department of Otolaryngology, Sunshine Union Hospital, Weifang, Shandong, China.
Department of Pediatrics, Sunshine Union Hospital, Weifang, Shandong, China.
Cell Cycle. 2023 Oct;22(20):2280-2287. doi: 10.1080/15384101.2023.2286802. Epub 2023 Dec 15.
Allergic rhinitis (AR) is very common in adolescents, and current treatment options are complex and unsatisfactory. The objective of this study was to analyze the association of lysyl oxidase (LOX) gene G473A polymorphism with susceptibility to AR in children. In addition, we analyzed the therapeutic effect of montelukast sodium on AR. Forty-five children with AR (research group, 8.16±2.88 years old) and 51 healthy children (control group, 8.22±3.87 years old) during the same period were selected. The LOX gene G473A polymorphism was detected with polymerase chain reaction (PCR)-restriction fragment length polymorphism method. The effect of G473A polymorphism in the occurrence of AR was assessed by logistic regression analysis. In addition, the levels of C-reactive protein (CRP), Interleukin (IL-6), and IL-8 were measured to observe the relationship between G473A polymorphism and inflammatory factors. Finally, montelukast sodium was given to children with AR to investigate the effect of G473A polymorphism on clinical outcomes. The number of G473A polymorphisms in the research group was not significantly different from the control group for GA-type ( = 0.521). However, the number of GG-type polymorphisms was less while the number of type AA was more than the control group ( = 0.044 and 0.046). Children carrying the AA gene had an approximately 4-fold increased risk of AR, while those carrying the GG gene had a decreased risk ( < 0.001). Moreover, children carrying the GG gene had lower levels of CRP, IL-6, and IL-8 and better clinical outcomes, while those carrying the AA gene had higher levels of inflammatory factors and worse outcomes (P<0.05). LOX gene G473A polymorphism is closely associated with AR pathogenesis and may have an important research value in antagonizing the therapeutic effect of montelukast sodium.
变应性鼻炎（AR）在青少年中非常常见，目前的治疗选择复杂且不尽如人意。本研究旨在分析赖氨酰氧化酶（LOX）基因 G473A 多态性与儿童 AR 易感性的关系。此外，我们还分析了孟鲁司特钠对 AR 的治疗效果。选择同期 45 例 AR 患儿（研究组，8.16±2.88 岁）和 51 例健康儿童（对照组，8.22±3.87 岁）。采用聚合酶链反应（PCR）-限制性片段长度多态性方法检测 LOX 基因 G473A 多态性。采用 logistic 回归分析评估 G473A 多态性在 AR 发生中的作用。此外，还检测了 C 反应蛋白（CRP）、白细胞介素（IL）-6 和 IL-8 的水平，以观察 G473A 多态性与炎症因子的关系。最后，给予 AR 患儿孟鲁司特钠，探讨 G473A 多态性对临床结局的影响。研究组 G473A 多态性的 GA 型数量与对照组无显著差异（ = 0.521）。然而，GG 型多态性数量较少，AA 型数量较多（ = 0.044 和 0.046）。携带 AA 基因的儿童患 AR 的风险增加约 4 倍，而携带 GG 基因的儿童患病风险降低（ < 0.001）。此外，携带 GG 基因的儿童 CRP、IL-6 和 IL-8 水平较低，临床结局较好，而携带 AA 基因的儿童炎症因子水平较高，结局较差（P<0.05）。LOX 基因 G473A 多态性与 AR 的发病机制密切相关，可能在拮抗孟鲁司特钠的治疗作用方面具有重要的研究价值。
