Shen Yang, Yuan Xiao-Dong, Hu Di, Ke Xia, Wang Xiao-Qiang, Hu Guo-Hua, Hong Su-Ling, Kang Hou-Yong
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China; Chongqing Key Laboratory of Ophthalmology, Chongqing, People's Republic of China.
Hum Immunol. 2014 Sep;75(9):991-5. doi: 10.1016/j.humimm.2014.07.004. Epub 2014 Jul 27.
Allergic rhinitis (AR) is an inflammatory disorder of the upper airway. Interleukin-27 (IL-27), a novel IL-12 family member, has recently been reported to play a role in some immune-related disorders. This study was performed to evaluate the potential association of IL-27 polymorphisms with AR in a Chinese Han population.
A case-control study was performed in 445 Chinese AR patients and 691 healthy controls. Three SNPs in the IL-27p28 gene, including rs153109, rs17855750 and rs181206, were detected using a polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP).
A significantly increased prevalence of the rs153109 TT genotype and the T allele was found in AR patients, while a decreased prevalence of the CT and CC genotypes and the C allele was found. For rs153109, the TT genotype and the T allele were significantly associated with the risk of AR, but the CT and CC genotypes and the C allele decreased the risk of AR; for rs17855750, the TT genotype and T allele were risk factors for AR, and the GT genotype and G allele provided protection. TTT and TTC haplotypes in the IL-27p28 gene were positively correlated with AR, while CGT, CTC and CTT haplotypes were associated with a significantly decreased risk of AR.
This study indicates that IL-27p28 polymorphisms rs153109 and rs17855750 are likely involved in AR susceptibility, making them potentially useful genetic biomarkers for AR susceptibility in the Chinese Han population.
变应性鼻炎（AR）是上呼吸道的一种炎症性疾病。白细胞介素-27（IL-27）是白细胞介素-12家族的一个新成员，最近有报道称其在一些免疫相关疾病中发挥作用。本研究旨在评估中国汉族人群中IL-27基因多态性与AR的潜在关联。
对445例中国AR患者和691例健康对照进行病例对照研究。采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）检测IL-27p28基因中的3个单核苷酸多态性（SNP），包括rs153109、rs17855750和rs181206。
在AR患者中发现rs153109的TT基因型和T等位基因的患病率显著增加，而CT和CC基因型以及C等位基因的患病率降低。对于rs153109，TT基因型和T等位基因与AR风险显著相关，但CT和CC基因型以及C等位基因降低了AR风险；对于rs17855750，TT基因型和T等位基因是AR的危险因素，而GT基因型和G等位基因提供了保护作用。IL-27p28基因中的TTT和TTC单倍型与AR呈正相关，而CGT、CTC和CTT单倍型与AR风险显著降低相关。
本研究表明，IL-27p28基因多态性rs153109和rs17855750可能与AR易感性有关，使其有可能成为中国汉族人群AR易感性的有用遗传生物标志物。
