Xia Xiaoyu, Li Han, Xu Xianbing, Wu Chao, Wang Zhenyu, Zhao Guanghua, Du Ming
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China.
Collaborative Innovation Centre of Provincial and Ministerial Co-construction for Seafood Deep Processing, Dalian, China.
J Sci Food Agric. 2024 Mar 30;104(5):2783-2791. doi: 10.1002/jsfa.13163. Epub 2024 Jan 2.
Lycopene (LYC), a carotenoid found in abundance in ripe red fruits, exhibits higher singlet oxygen quenching activity than other carotenoids. However, the stability of LYC is extremely poor due to its high double-bond content. In this paper, a nano-encapsulation strategy based on highly stable marine-derived ferritin GF1 nanocages was used to improve the thermal stability and oxidation resistance of LYC, thereby boosting its functional effectiveness and industrial applicability.
The preparation of GF1-LYC nanoparticles benefited from the pH-responsive reversible self-assembly of GF1 to capture LYC molecules into GF1 cavities with a LYC-to-protein ratio of 51 to 1. After the encapsulation of the LYC, the reassembled GF1 nanocages maintained intact morphology and good monodispersity. The GF1-LYC nanoparticles incorporated the characteristic LYC peaks in spectrograms, and their powder form contained the crystalline form of LYC. Molecular docking revealed that LYC bound with the inner triple-axis channel areas of GF1, interacting with VAL139, LYS72, LYS65, TYR69, PHE129, HIS133, HIS62, and TYR134 amino acids through hydrophobic bonds. Fourier transform infrared spectroscopy also demonstrated the bonding of GF1 and LYC. In comparison with free LYC, GF1 reduced the thermal degradation of encapsulated LYC at 37 °C significantly and maintained the 2,2-Diphenyl-1-picrylhydrazyl (DPPH)-scavenging ability of LYC.
As expected, the water solubility, thermal stability, and antioxidant capacity of encapsulated LYC from GF1-LYC nanoparticles was notably improved in comparison with free LYC, indicating that the shell-like marine ferritin nanoplatform might enhance the stable delivery of LYC and promote its utilization in the field of food nutrition and in other industries. © 2023 Society of Chemical Industry.
番茄红素（LYC）是一种在成熟红色果实中大量存在的类胡萝卜素，其单线态氧猝灭活性高于其他类胡萝卜素。然而，由于其高双键含量，LYC的稳定性极差。本文采用基于高度稳定的海洋来源铁蛋白GF1纳米笼的纳米封装策略，以提高LYC的热稳定性和抗氧化性，从而增强其功能有效性和工业适用性。
GF1-LYC纳米颗粒的制备得益于GF1的pH响应可逆自组装，以51:1的LYC与蛋白质比例将LYC分子捕获到GF1腔内。LYC封装后，重新组装的GF1纳米笼保持完整形态和良好的单分散性。GF1-LYC纳米颗粒在光谱图中呈现出LYC的特征峰，其粉末形式含有LYC的晶体形式。分子对接表明，LYC与GF1的内部三轴通道区域结合，通过疏水键与VAL139、LYS72、LYS65、TYR69、PHE129、HIS133、HIS62和TYR134氨基酸相互作用。傅里叶变换红外光谱也证明了GF1与LYC的结合。与游离LYC相比，GF1显著降低了37°C下封装LYC的热降解，并保持了LYC的2,2-二苯基-1-苦基肼（DPPH）清除能力。
正如预期的那样，与游离LYC相比，GF1-LYC纳米颗粒中封装的LYC的水溶性、热稳定性和抗氧化能力显著提高，表明壳状海洋铁蛋白纳米平台可能增强LYC的稳定递送，并促进其在食品营养领域和其他行业的应用。©2023化学工业协会。
