Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Johannesburg, South Africa.
J Biophotonics. 2024 Feb;17(2):e202300230. doi: 10.1002/jbio.202300230. Epub 2023 Dec 4.
Treatment of chronic diabetic wounds is an ongoing socio-economic challenge. Dysregulated signalling pathways characterise cells from chronic diabetic wounds. Photobiomodulation (PBM) stimulates healing by eliciting photochemical effects that affect gene regulation. JAK/STAT signalling is a primary signal transduction pathway involved in wound healing. This in vitro study aimed to determine if PBM at 830 nm and a fluence of 5 J/cm regulates genes related to JAK/STAT signalling in wounded and diabetic wounded fibroblast cells. A continuous wave diode laser (12.53 mW/cm ) was used to irradiate cells. Forty-eight hours post-PBM, RT-qPCR was used to analyse 84 genes related to JAK/STAT signalling. Five genes were upregulated and four downregulated in wounded cell models, while six genes were downregulated in diabetic wounded models. The results show drastic gene expression differences between wounded and diabetic wounded cell models in response to PBM using 830 nm.
治疗慢性糖尿病性伤口是一个持续存在的社会经济挑战。慢性糖尿病性伤口的细胞表现出失调的信号通路。光生物调节（PBM）通过引发影响基因调控的光化学效应来刺激愈合。JAK/STAT 信号转导是参与伤口愈合的主要信号转导途径。这项体外研究旨在确定 830nm 的 PBM 和 5J/cm 的剂量是否调节创伤和糖尿病创伤成纤维细胞中与 JAK/STAT 信号相关的基因。使用连续波二极管激光器（12.53mW/cm）照射细胞。在 PBM 后 48 小时，使用 RT-qPCR 分析与 JAK/STAT 信号相关的 84 个基因。在创伤细胞模型中，有 5 个基因上调，4 个基因下调，而在糖尿病创伤模型中，有 6 个基因下调。结果表明，在使用 830nm 的 PBM 时，创伤和糖尿病创伤细胞模型之间的基因表达存在明显差异。
