Houreld Nicolette N, Ayuk Sandra M, Abrahamse Heidi
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.
Cells. 2018 Apr 16;7(4):30. doi: 10.3390/cells7040030.
Diabetes affects extracellular matrix (ECM) metabolism, contributing to delayed wound healing and lower limb amputation. Application of light (photobiomodulation, PBM) has been shown to improve wound healing. This study aimed to evaluate the influence of PBM on cell adhesion molecules (CAMs) in diabetic wound healing. Isolated human skin fibroblasts were grouped into a diabetic wounded model. A diode laser at 660 nm with a fluence of 5 J/cm² was used for irradiation and cells were analysed 48 h post-irradiation. Controls consisted of sham-irradiated (0 J/cm²) cells. Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of CAM-related genes. Ten genes were up-regulated in diabetic wounded cells, while 25 genes were down-regulated. Genes were related to transmembrane molecules, cell–cell adhesion, and cell–matrix adhesion, and also included genes related to other CAM molecules. PBM at 660 nm modulated gene expression of various CAMs contributing to the increased healing seen in clinical practice. There is a need for new therapies to improve diabetic wound healing. The application of PBM alongside other clinical therapies may be very beneficial in treatment.
糖尿病会影响细胞外基质（ECM）代谢，导致伤口愈合延迟和下肢截肢。光疗法（光生物调节作用，PBM）已被证明可促进伤口愈合。本研究旨在评估PBM对糖尿病伤口愈合过程中细胞黏附分子（CAMs）的影响。将分离出的人皮肤成纤维细胞分组建立糖尿病伤口模型。使用波长660 nm、能量密度为5 J/cm²的二极管激光进行照射，并在照射后48小时对细胞进行分析。对照组为假照射（0 J/cm²）的细胞。采用实时逆转录（RT）定量聚合酶链反应（qPCR）来测定CAM相关基因的表达。在糖尿病伤口细胞中有10个基因上调，25个基因下调。这些基因与跨膜分子、细胞间黏附以及细胞与基质黏附有关，还包括与其他CAM分子相关的基因。660 nm的PBM调节了各种CAMs的基因表达，这有助于在临床实践中观察到的愈合增强。需要新的疗法来改善糖尿病伤口愈合。将PBM与其他临床疗法联合应用可能对治疗非常有益。
