Comparison of glutathione S-transferases in mouse, guinea pig, rabbit and hamster liver cytosol to those in rat liver.

The glutathione S-transferases (GSTs) of hepatic cytosol of rats, mice, guinea pigs, rabbits and hamsters were simultaneously investigated with respect to substrate specificity, subunit composition and elution profile of GSTs. The activity towards 1-chloro-2,4-dinitrobenzene was the highest in hamsters, followed by rabbits, guinea pigs, mice and rats. On the analysis of SDS-PAGE, Ya subunit band of GST was detected in only rats, not others. There was also seen a marked species difference in elution profile of GST activity on S-sepharose column. The elution patterns from rats, mice and rabbits were quite different from hamsters and guinea pigs.