Smith G J, Ohl V S, Litwack G
Cancer Res. 1980 Jun;40(6):1787-90.
Glutathione S-transferases have been purified to homogeneity from Chinese hamster liver. Three enzyme forms were separated and designated Forms I, II, and III in order of their elution from carboxymethylcellulose columns. The forms exhibit close physical similarities to glutathione S-transferases B (ligandin) of rat liver and epsilon of humam liver. However, enzyme kinetic analysis indicates that the hamster enzymes exhibit similar Km values but higher Vmax values towards common substrates compared with the rat and human forms. These differences, which explain the increased enzymic activities of hamster glutathione S-transferases in vivo and in vitro, appear to be related to slight differences in the peptide composition of hamster liver glutathione S-transferases compared to the rat and human enzymes.
谷胱甘肽S-转移酶已从中国仓鼠肝脏中纯化至同质。分离出三种酶形式，并按照它们从羧甲基纤维素柱上洗脱的顺序分别命名为形式I、形式II和形式III。这些形式与大鼠肝脏的谷胱甘肽S-转移酶B（配体蛋白）和人类肝脏的ε形式在物理性质上非常相似。然而，酶动力学分析表明，与大鼠和人类的形式相比，仓鼠的酶对常见底物表现出相似的Km值，但Vmax值更高。这些差异解释了仓鼠谷胱甘肽S-转移酶在体内和体外酶活性增加的原因，似乎与仓鼠肝脏谷胱甘肽S-转移酶与大鼠和人类酶相比在肽组成上的细微差异有关。
