New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation (KMEDI-Hub), Daegu, Korea.
Department of Biology, College of Natural Science, Kyungpook National University, Daegu, Korea.
Exp Brain Res. 2024 Jan;242(1):257-265. doi: 10.1007/s00221-023-06742-2. Epub 2023 Nov 27.
The purpose of the study was to which investigate whether dexamethasone, which has anti-inflammatory and immune response suppression roles, could treat noise-induced hearing loss caused by damage to hair cells in the cochlea. The experiment used 8-week-old CBA mice exposed to white noise at an intensity of 110 dB SPL for 2 h, with hearing loss confirmed by the auditory brainstem response test. Dexamethasone was administered by intraperitoneal injection for 5 days, and the therapeutic effect was investigated for 3 weeks. The experimental groups were 3 mg/kg of dexamethasone (3 mpk) and 10 mg/kg of dexamethasone (10 mpk), and the control group was a saline-administered group. The results showed that compared to the control group, the hearing threshold value was recovered by 10 dB SPL compared to the saline group from the 14th day in the 3 mpk group. In the 10 mpk group, thresholds were recovered from the 7th day compared to the saline group. This difference was similar at 4 kHz, and in the case of the 10 mpk group, the threshold was recovered by 20 dB SPL compared to the saline group. The study also confirmed the restoration of nerve cell activity and showed a recovery effect of about 20 µV in the amplitude value change in the 10 mpk group. In conclusion, the study suggests that dexamethasone has a therapeutic effect for noise-induced hearing loss by increasing the activity of nerve cells and showing a recovery effect from hair cells damaged by noise.
本研究旨在探讨地塞米松（一种具有抗炎和免疫抑制作用的药物）是否可以治疗因耳蜗毛细胞损伤引起的噪声性听力损失。实验使用 8 周龄的 CBA 小鼠，在 110dB SPL 的白噪声环境中暴露 2 小时，通过听觉脑干反应测试确认听力损失。地塞米松通过腹腔注射给药 5 天，并在 3 周内观察治疗效果。实验组分别给予 3mg/kg 地塞米松（3mpk）和 10mg/kg 地塞米松（10mpk），对照组给予生理盐水处理。结果显示，与对照组相比，3mpk 组在第 14 天的听力阈值比生理盐水组恢复了 10dB SPL，10mpk 组从第 7 天开始比生理盐水组恢复了阈值。在 4kHz 时，这种差异相似，并且在 10mpk 组中，与生理盐水组相比，阈值恢复了 20dB SPL。该研究还证实了神经细胞活性的恢复，并显示了 10mpk 组的幅度值变化约为 20µV 的恢复效应。综上所述，本研究表明地塞米松通过增加神经细胞的活性并显示出对噪声损伤的毛细胞的恢复效应，对噪声性听力损失具有治疗作用。
